Vitamin B6 and the immunity in kidney transplant recipients.

Abstract

OBJECTIVE:

The study aimed to determine vitamin B6 status in elderly (age ≥ 60 years) and younger (age <60 years) recipients of allogeneic kidney graft and to investigate associations between vitamin B6 status and immunity markers.

DESIGN:

A retrospective observational study.

SETTING:

The study was conducted at the Medical University of Gdańsk, Poland.

SUBJECTS:

We recruited 34 kidney allograft recipients (17 males and 17 females) and allocated them into 2 groups: patients aged ≥ 60 years (18 patients) and those aged <60 years (16 patients). Exclusion criteria included patients receiving vitamin B6 supplementation or drugs known to influence vitamin B6 metabolism.

MAIN OUTCOME MEASURE:

Plasma levels of pyridoxal 5'-phosphate (PLP), pyridoxal, pyridoxine, pyridoxamine, pyridoxamine 5'-phosphate, and 4 pyridoxic acid were determined by high-performance liquid chromatography. Measured immunity markers were serum cytokines (interleukin-6, interleukin-10, and transforming growth factor-β), levels of T-lymphocyte subsets, and the proliferative ability of peripheral blood mononuclear cells.

RESULTS:

Concentrations of all vitamin B6 vitamers in plasma (PLP, pyridoxal, pyridoxamine 5'-phosphate, pyridoxamine, pyridoxine, 4 pyridoxic acid) were comparable in the 2 studied groups. There were no cases of PLP deficiency in the study population, but 29% of patients had PLP concentrations more than the upper reference limit. Vitamin B6 vitamer concentrations were not influenced by gender, estimated glomerular filtration rate, and circulating phosphate concentration. There was no difference in immunity markers according to age. However, the plasma concentrations of vitamin B6 vitamers were inversely associated with levels of CD28(+) lymphocyte subsets, as well as with the proliferative response of peripheral blood mononuclear cells in both groups.

CONCLUSIONS:

No cases of vitamin B6 deficiency were found among kidney allograft recipients, and we report inverse links between vitamin B6 vitamer concentrations and markers of cellular immunity, suggesting that bioactive vitamin B6 concentration in kidney allograft recipients merits further investigation.

• Authors: Magdalena Jankowska, Marcin Marszałł, Alicja Dębska-Ślizień, Juan J Carrero, Bengt Lindholm, Wojciech Czarnowski, Bolesław Rutkowski, Piotr Trzonkowski
• Journal: Journal of renal nutrition : the official journal of the Council on Renal Nutrition of the National Kidney Foundation (J Ren Nutr) Vol. 23 Issue 1 Pg. 57-64 (Jan 2013) ISSN: 1532-8503 [Electronic] United States
• PMID: 22445054 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2013 National Kidney Foundation, Inc. Published by Elsevier Inc. All rights reserved.

Chemical References

• Biomarkers
• Interleukin-6
• Latent TGF-beta Binding Proteins
• Ltbp1 protein, rat
• Interleukin-10
• Pyridoxal
• Pyridoxic Acid
• Pyridoxamine
• Vitamin B 6
• Pyridoxine

Topics

• Adolescent
• Adult
• Aged
• Biomarkers (blood)
• Child
• Chromatography, High Pressure Liquid
• Female
• Humans
• Immunity (immunology)
• Interleukin-10 (blood)
• Interleukin-6 (blood)
• Kidney Transplantation
• Latent TGF-beta Binding Proteins (blood)
• Leukocytes, Mononuclear (metabolism)
• Male
• Middle Aged
• Pyridoxal (blood)
• Pyridoxamine (blood)
• Pyridoxic Acid (blood)
• Pyridoxine (blood)
• Retrospective Studies
• T-Lymphocyte Subsets (metabolism)
• Vitamin B 6 (blood)
• Vitamin B 6 Deficiency (blood)
• Young Adult