Chemiluminescence enzyme immunoassay using magnetic nanoparticles for detection of neuron specific enolase in human serum.

To detect a biomarker for small cell lung carcinoma, neuron specific enolase (NSE), a sensitive and specific chemiluminescence enzyme immunoassay was developed. Fluorescein isothiocyanate (FITC) labeled NSE capture antibody connected with NSE and alkaline phosphatase (ALP) labeled NSE detection antibody in a sandwich-type detection manner. This immune complex was further reacted with anti-FITC coated magnetic beads. In a magnetic field, the complex was enriched, and the sensitivity was thus enhanced. The limit of detection (LOD) of this method was <0.2 ng mL(-1). The proposed immunoassay was highly selective, and not interfered by hook effect. The recovery was >83.0% and the coefficient of variation was <10.0%. Human sera from 120 patients were tested with the presented and traditional chemiluminescence enzyme immunoassay. An excellent linear relationship was obtained between two techniques. Overall, this immunoassay offers a promising alternative for NSE detection than traditional clinical examinations.
AuthorsXiaoling Fu, Meng Meng, Yu Zhang, Yongmei Yin, Xiangsheng Zhang, Rimo Xi
JournalAnalytica chimica acta (Anal Chim Acta) Vol. 722 Pg. 114-8 (Apr 13 2012) ISSN: 1873-4324 [Electronic] Netherlands
PMID22444542 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright © 2012 Elsevier B.V. All rights reserved.
Chemical References
  • Antibodies, Immobilized
  • Biomarkers, Tumor
  • Enzymes, Immobilized
  • Magnetite Nanoparticles
  • Alkaline Phosphatase
  • Phosphopyruvate Hydratase
  • Fluorescein-5-isothiocyanate
  • Alkaline Phosphatase (immunology, metabolism)
  • Antibodies, Immobilized (immunology)
  • Biomarkers, Tumor (blood)
  • Enzymes, Immobilized (metabolism)
  • Fluorescein-5-isothiocyanate (chemistry)
  • Humans
  • Immunoenzyme Techniques
  • Luminescent Measurements
  • Lung Neoplasms (diagnosis)
  • Magnetite Nanoparticles (chemistry)
  • Phosphopyruvate Hydratase (blood, immunology)

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