Arylamine N-acetyltransferase activities in cell lines of mouse, rat, hamster and man differing in their sensitivity to 1,6-dinitropyrene.

Abstract

This study was aimed at monitoring N-acetyltransferase activities of continuous cell lines, which differ in their sensitivity to the toxic effects of nitroaromatic compounds. Transferase activities were measured toward the acetyl acceptors sulfamethazine and p-aminobenzoic acid in partially purified preparation of cytosols. Cell lines such as hamster V79, BHK, rat hepatoma H4IIEC3G- or fibroblast 208F, which are sensitive to 1,6-dinitropyrene (1,6-DNP), possess high transferase activities ranging from 120-270 nmol/min x mg protein. In contrast, human lung cells NCI-H322, mouse and rat hepatoma cells BW1J and H5, respectively, which are resistant to 1,6-DNP contain no or low transferase activity of less than 15 nmol/min x mg. There was no apparent correlation between 1,6-DNP sensitivity and acetyltransferase levels in a few cell lines, e.g. rat hepatoma HTC, 2sFou and 5L, which express intermediate transferase activities ranging from 25-50 nmol/min x mg protein. The results suggest that acetylation is an essential step in activating 1,6-DNP to toxic products in mammalian cells.

• Authors: A K Srivastava, F J Wiebel
• Journal: Toxicology letters (Toxicol Lett) Vol. 54 Issue 1 Pg. 71-6 (Nov 1990) ISSN: 0378-4274 [Print] Netherlands
• PMID: 2244342 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Pyrenes
• 1,6-dinitropyrene
• Arylamine N-Acetyltransferase

Topics

• Animals
• Arylamine N-Acetyltransferase (metabolism)
• Cell Line (drug effects)
• Cells, Cultured
• Cricetinae
• Humans
• Liver Neoplasms, Experimental (enzymology)
• Mice
• Pyrenes (toxicity)
• Rats
• Species Specificity