Chloride intracellular channel 1 is an important factor in the lymphatic metastasis of hepatocarcinoma.

Abstract

We have previously demonstrated that chloride intracellular channel 1 (CLIC1) is involved in the lymphatic metastasis of tumors. In this study, a self-designed shRNA sequence of mouse CLIC1 gene was synthesized and inserted into a pGPU6/GFP/Neo plasmid, then stably transfected into mouse hepatic carcinoma cell line Hca-F cells to down-regulate the expression of CLIC1 gene. The levels of expression of CLIC1 mRNA and protein were detected by real-time quantitative polymerase chain reaction (qRT-PCR) and western blot (WB) analysis, respectively. The down-regulation of CLIC1 enhanced proliferative activity, increased the ratio of G2/M and decreased percentage of apoptosis. In addition, the capability of migration and invasion decreased significantly. The results indicate that CLIC1 is a critical factor in the development of lymphatic metastasis.

• Authors: Rong-Kuan Li, Jun Zhang, Yu-Hong Zhang, Miao-Ling Li, Mei Wang, Jian-Wu Tang
• Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie (Biomed Pharmacother) Vol. 66 Issue 3 Pg. 167-72 (Apr 2012) ISSN: 1950-6007 [Electronic] France
• PMID: 22440897 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2012 Elsevier Masson SAS. All rights reserved.

Chemical References

• Chloride Channels
• Clic1 protein, mouse
• RNA, Messenger
• RNA, Small Interfering

Topics

• Animals
• Apoptosis (genetics)
• Carcinoma, Hepatocellular (genetics, pathology)
• Cell Division (genetics)
• Cell Growth Processes (genetics)
• Cell Line, Tumor
• Cell Movement (genetics)
• Chloride Channels (genetics)
• Down-Regulation
• G2 Phase (genetics)
• Liver Neoplasms (genetics, pathology)
• Lymphatic Metastasis
• Mice
• Neoplasm Invasiveness (genetics)
• RNA, Messenger (genetics)
• RNA, Small Interfering (genetics)
• Transfection (methods)