Sensitive colorimetric visualization of dihydronicotinamide adenine dinucleotide based on anti-aggregation of gold nanoparticles via boronic acid-diol binding.

Abstract

A facile, highly sensitive colorimetric strategy for dihydronicotinamide adenine dinucleotide (NADH) detection is proposed based on anti-aggregation of gold nanoparticles (AuNPs) via boronic acid-diol binding chemistry. The aggregation agent, 4-mercaptophenylboronic acid (MPBA), has specific affinity for AuNPs through Au-S interaction, leading to the aggregation of AuNPs by self-dehydration condensation at a certain concentration, which is responsible for a visible color change of AuNPs from wine red to blue. With the addition of NADH, MPBA would prefer reacting with NADH to form stable borate ester via boronic acid-diol binding dependent on the pH and solvent, revealing an obvious color change from blue to red with increasing the concentration of NADH. The anti-aggregation effect of NADH on AuNPs was seen by the naked eye and monitored by UV-vis extinction spectra. The linear range of the colorimetric sensor for NADH is from 8.0 × 10(-9)M to 8.0 × 10(-6)M, with a low detection limit of 2.0 nM. The as-established colorimetric strategy opened a new avenue for NADH determination.

• Authors: Shufeng Liu, Zongfeng Du, Peng Li, Feng Li
• Journal: Biosensors & bioelectronics (Biosens Bioelectron) Vol. 35 Issue 1 Pg. 443-446 (May 15 2012) ISSN: 1873-4235 [Electronic] England
• PMID: 22425223 (Publication Type: Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2012 Elsevier B.V. All rights reserved.

Chemical References

• 4-mercaptophenylboronic acid
• Boronic Acids
• Sulfhydryl Compounds
• NAD
• Gold

Topics

• Biosensing Techniques (methods, statistics & numerical data)
• Boronic Acids
• Colorimetry (methods, statistics & numerical data)
• Gold
• Limit of Detection
• Metal Nanoparticles
• NAD (analysis)
• Sensitivity and Specificity
• Sulfhydryl Compounds