Anti-inflammatory activities of a kolaviron-inhibition of nitric oxide, prostaglandin E2 and tumor necrosis factor-alpha production in activated macrophage-like cell line.

Abstract

Kolaviron (KV), a biflavonoid fraction from the seeds of Garcinia kola has been shown to posess antiinflammatory properties in animal models of inflammation. In this study, the effect of KV on carrageenan-induced paw edema was investigated in mice. Furthermore, the effects of KV on the production of the pro-inflammatory mediators- nitric oxide (NO) and tumor necrosis factor alpha (TNF-alpha) were examined in an activated macrophage-like cell lines, RAW 264.7 cells. Administration of KV prior to injection of carrageenan significantly reduced the paw inflammation in a dose-dependent manner. KV consistently inhibited in-vitro production of NO and secretion of TNF-alpha in a dose-dependent manner. In addition, KV reduced the production of PGE2 in LPS-stimulated macrophages. Viability of cells at all concentrations studied was unaffected as determined MTT [3-(4,5- dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide] cytotoxicity assay. These results suggest that KV has inhibitory effects on LPS-induced TNF-alpha, NO and PGE2 production.

• Authors: S B Olaleye, S A Onasanwo, A O Ige, K K Wu, C H Cho
• Journal: African journal of medicine and medical sciences (Afr J Med Med Sci) Vol. 39 Suppl Pg. 41-6 (Dec 2010) ISSN: 0309-3913 [Print] Nigeria
• PMID: 22416643 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anti-Inflammatory Agents
• Flavonoids
• Plant Extracts
• Tumor Necrosis Factor-alpha
• Nitric Oxide
• Carrageenan
• Dinoprostone
• kolaviron

Topics

• Animals
• Anti-Inflammatory Agents (isolation & purification, pharmacology)
• Carrageenan (administration & dosage, pharmacology)
• Cell Line
• Dinoprostone (antagonists & inhibitors, biosynthesis, metabolism)
• Dose-Response Relationship, Drug
• Edema (drug therapy, etiology)
• Enzyme-Linked Immunosorbent Assay
• Flavonoids (pharmacology)
• Garcinia kola (chemistry)
• Macrophages (drug effects, metabolism)
• Mice
• Nitric Oxide (antagonists & inhibitors, biosynthesis, metabolism)
• Phytotherapy
• Plant Extracts (isolation & purification, pharmacology)
• Seeds (chemistry)
• Treatment Outcome
• Tumor Necrosis Factor-alpha (antagonists & inhibitors, biosynthesis, metabolism)