Ethanol extract from a Chinese herbal formula, "Zuojin Pill", inhibit the expression of inflammatory mediators in lipopolysaccharide-stimulated RAW 264.7 mouse macrophages.
Abstract | ETHNOPHARMACOLOGICAL RELEVANCE:
Zuojin Pill (ZJP), a traditional Chinese medicinal decoction that has been used in treating gastritis, gastric ulcer since 15th century, contains two herbs: Rhizoma Coptidis and Fructus Evodiae in the ratio of 6:1 (w/w). Alkaloids are the main active principles contributing to ZJP's efficacy, but anti-inflammatory mechanism has not been fully clarified. AIM OF THE STUDY: The objective of the study is to reveal anti-inflammatory molecular mechanism of ethanol extract from ZJP, which would form an additional proof to the traditional experience of ZJP in clinical administration. MATERIALS AND METHODS: RESULTS: The secretions of NO, PGE(2) and the mRNA expression of iNOS, COX-2 were significantly inhibited, moreover, the protein and mRNA expressions of IL-6, IL-1β and TNF-α were inhibited by preventing the nuclear translocation of the NF-κB p50 and p65 subunits. The proteome profiler array showed that 15 cytokines and chemokines involved in the inflammatory process were down-regulated by ZJP. CONCLUSION: These results suggest that the anti-inflammatory properties of ethanol extract from ZJP might be the results from the inhibition of iNOS, COX-2, IL-6, IL-1β, and TNF-α expression through preventing the nuclear translocation of the NF-κB p50 and p65 subunits in RAW 264.7 cells. In addition, these results provided evidence to understand the therapeutic effects of ZJP on gastritis, gastric ulcer, and other inflammatory diseases in clinic.
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Authors | Qiang-Song Wang, Yuan-Lu Cui, Tian-Jiao Dong, Xin-Fang Zhang, Ke-Ming Lin |
Journal | Journal of ethnopharmacology
(J Ethnopharmacol)
Vol. 141
Issue 1
Pg. 377-85
(May 07 2012)
ISSN: 1872-7573 [Electronic] Ireland |
PMID | 22414473
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2012 Elsevier Ireland Ltd. All rights reserved. |
Chemical References |
- Alkaloids
- Anti-Inflammatory Agents
- Cytokines
- Drugs, Chinese Herbal
- Inflammation Mediators
- Lipopolysaccharides
- NF-kappa B p50 Subunit
- RNA, Messenger
- Rela protein, mouse
- Solvents
- Transcription Factor RelA
- zuojin
- Nitric Oxide
- Ethanol
- Nitric Oxide Synthase Type II
- Nos2 protein, mouse
- Ptgs2 protein, mouse
- Cyclooxygenase 2
- Dinoprostone
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Topics |
- Alkaloids
(chemistry, pharmacology)
- Animals
- Anti-Inflammatory Agents
(chemistry, pharmacology)
- Cell Line
- Cell Survival
(drug effects)
- Chromatography, High Pressure Liquid
- Colorimetry
- Cyclooxygenase 2
(genetics, metabolism)
- Cytokines
(genetics, metabolism)
- Dinoprostone
(metabolism)
- Dose-Response Relationship, Drug
- Drugs, Chinese Herbal
(chemistry, pharmacology)
- Enzyme-Linked Immunosorbent Assay
- Ethanol
(chemistry)
- Inflammation Mediators
(metabolism)
- Lipopolysaccharides
(pharmacology)
- Macrophages
(drug effects, immunology)
- Mice
- NF-kappa B p50 Subunit
(genetics, metabolism)
- Nitric Oxide
(metabolism)
- Nitric Oxide Synthase Type II
(genetics, metabolism)
- Phytotherapy
- Plants, Medicinal
- Protein Array Analysis
- Protein Transport
- Proteomics
(methods)
- RNA, Messenger
(metabolism)
- Real-Time Polymerase Chain Reaction
- Reverse Transcriptase Polymerase Chain Reaction
- Solvents
(chemistry)
- Transcription Factor RelA
(genetics, metabolism)
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