IL-9 induces VEGF secretion from human mast cells and IL-9/IL-9 receptor genes are overexpressed in atopic dermatitis.

Abstract

Interleukin 9 (IL-9) has been implicated in mast cell-related inflammatory diseases, such as asthma, where vascular endothelial growth factor (VEGF) is involved. Here we report that IL-9 (10-20 ng/ml) induces gene expression and secretion of VEGF from human LAD2. IL-9 does not induce mast cell degranulation or the release of other mediators (IL-1, IL-8, or TNF). VEGF production in response to IL-9 involves STAT-3 activation. The effect is inhibited (about 80%) by the STAT-3 inhibitor, Stattic. Gene-expression of IL-9 and IL-9 receptor is significantly increased in lesional skin areas of atopic dermatitis (AD) patients as compared to normal control skin, while serum IL-9 is not different from controls. These results imply that functional interactions between IL-9 and mast cells leading to VEGF release contribute to the initiation/propagation of the pathogenesis of AD, a skin inflammatory disease.

• Authors: Nikolaos Sismanopoulos, Danae A Delivanis, Konstantinos D Alysandratos, Asimenia Angelidou, Magdalini Vasiadi, Anastasia Therianou, Theoharis C Theoharides
• Journal: PloS one (PLoS One) Vol. 7 Issue 3 Pg. e33271 ( 2012) ISSN: 1932-6203 [Electronic] United States
• PMID: 22413008 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)

Chemical References

• Interleukin-9
• RNA, Messenger
• Receptors, Interleukin-9
• STAT3 Transcription Factor
• Vascular Endothelial Growth Factor A

Topics

• Cell Line
• Dermatitis, Atopic (genetics, immunology, metabolism)
• Humans
• Interleukin-9 (blood, genetics, pharmacology)
• Mast Cells (drug effects, immunology, metabolism)
• Phosphorylation
• RNA, Messenger (genetics, metabolism)
• Receptors, Interleukin-9 (genetics)
• STAT3 Transcription Factor (metabolism)
• Vascular Endothelial Growth Factor A (metabolism)