Prostaglandin E(2) produced by the lung augments the effector phase of allergic inflammation.

Abstract

Elevated PGE(2) is a hallmark of most inflammatory lesions. This lipid mediator can induce the cardinal signs of inflammation, and the beneficial actions of nonsteroidal anti-inflammatory drugs are attributed to inhibition of cyclooxygenase (COX)-1 and COX-2, enzymes essential in the biosynthesis of PGE(2) from arachidonic acid. However, both clinical studies and rodent models suggest that, in the asthmatic lung, PGE(2) acts to restrain the immune response and limit physiological change secondary to inflammation. To directly address the role of PGE(2) in the lung, we examined the development of disease in mice lacking microsomal PGE(2) synthase-1 (mPGES1), which converts COX-1/COX-2-derived PGH(2) to PGE(2). We show that mPGES1 determines PGE(2) levels in the naive lung and is required for increases in PGE(2) after OVA-induced allergy. Although loss of either COX-1 or COX-2 increases the disease severity, surprisingly, mPGES1(-/-) mice show reduced inflammation. However, an increase in serum IgE is still observed in the mPGES1(-/-) mice, suggesting that loss of PGE(2) does not impair induction of a Th2 response. Furthermore, mPGES1(-/-) mice expressing a transgenic OVA-specific TCR are also protected, indicating that PGE(2) acts primarily after challenge with inhaled Ag. PGE(2) produced by the lung plays the critical role in this response, as loss of lung mPGES1 is sufficient to protect against disease. Together, this supports a model in which mPGES1-dependent PGE(2) produced by populations of cells native to the lung contributes to the effector phase of some allergic responses.

• Authors: Rachel J Church, Leigh A Jania, Beverly H Koller
• Journal: Journal of immunology (Baltimore, Md. : 1950) (J Immunol) Vol. 188 Issue 8 Pg. 4093-102 (Apr 15 2012) ISSN: 1550-6606 [Electronic] United States
• PMID: 22412193 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)

Chemical References

• Cytokines
• Membrane Proteins
• Receptors, Antigen, T-Cell
• Ovalbumin
• Ptgs2 protein, mouse
• Cyclooxygenase 1
• Cyclooxygenase 2
• Ptgs1 protein, mouse
• Intramolecular Oxidoreductases
• Prostaglandin-E Synthases
• Dinoprostone

Topics

• Animals
• Cell Proliferation
• Cyclooxygenase 1 (deficiency, genetics, immunology)
• Cyclooxygenase 2 (deficiency, genetics, immunology)
• Cytokines (biosynthesis, immunology)
• Dinoprostone (immunology, metabolism)
• Female
• Hypersensitivity (immunology, metabolism, pathology)
• Inflammation (immunology, metabolism, pathology)
• Intramolecular Oxidoreductases (deficiency, genetics, immunology)
• Lung (immunology, metabolism, pathology)
• Male
• Membrane Proteins (deficiency, genetics, immunology)
• Mice
• Mice, Inbred C57BL
• Mice, Knockout
• Ovalbumin
• Prostaglandin-E Synthases
• Receptors, Antigen, T-Cell (genetics, immunology)
• Th2 Cells (immunology, metabolism)