Inhibition of inflammations and macrophage activation by ginsenoside-Re isolated from Korean ginseng (Panax ginseng C.A. Meyer).

Abstract

This study was undertaken to evaluate the effect of ginsenoside-Re (Gin-Re) isolated from roots of Panax ginseng on carrageenan-induced paw and TPA-induced skin inflammations in experimental mice. Moreover, to confirm further the anti-inflammatory activities of Gin-Re, LPS-induced macrophage activation model was also used. Exposure of TPA on the ear of BALB/c mice caused a marked increase in both ear thickness and skin water content. Gin-Re caused significant decrease in ear thickness and subsequently reduced the water content compared to only TPA treated group (p<0.05). Furthermore, histological analysis clearly confirmed that Gin-Re inhibited the inflammatory responses of skin inflammation in animal model. Gin-Re was responded well in inhibiting paw thickness, MDA level and also NO level in carrageenan induced paw edema model compared to only carrageenan treated group. Treatment with Gin-Re inhibited secretion levels of inflammatory mediators such as tumor necrosis factor α (TNF α), and interleukin-1β (IL-1β) in LPS-stimulated murine macrophage Raw 264.7 cells. Despite the fact that Gin-Re has weaker anti-inflammatory potential than the positive controls, indomethacin and hydrocortisone, in the entire group tested, quite effective anti-inflammatory activity was shown by Gin-Re, which could be helpful to develop medicinal preparations for various inflammatory diseases.

• Authors: Souren Paul, Heung Sop Shin, Sun Chul Kang
• Journal: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association (Food Chem Toxicol) Vol. 50 Issue 5 Pg. 1354-61 (May 2012) ISSN: 1873-6351 [Electronic] England
• PMID: 22401937 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2012 Elsevier Ltd. All rights reserved.

Chemical References

• Ginsenosides
• ginsenoside Re

Topics

• Animals
• Cell Line
• Enzyme-Linked Immunosorbent Assay
• Ginsenosides (pharmacology)
• Inflammation (metabolism, prevention & control)
• Macrophage Activation (drug effects)
• Mice
• Mice, Inbred BALB C
• Panax (chemistry)