Abstract |
Newcastle disease virus (NDV) can replicate in tumor cells and induce apoptosis in late stages of infection. However, the interaction between NDV and cells in early stages of infection is not well understood. Here, we report that, shortly after infection, NDV triggers the formation of autophagosomes in U251 glioma cells, as demonstrated by an increased number of double-membrane vesicles, GFP- microtubule-associated protein 1 light chain 3 (GFP-LC3) a dot formations, and elevated production of LC3II. Moreover, modulation of NDV-induced autophagy by rapamycin, chloroquine or small interfering RNAs targeting the genes critical for autophagosome formation (Atg5 and Beclin-1) affects virus production, indicating that autophagy may be utilized by NDV to facilitate its own production. Furthermore, the class III phosphatidylinositol 3-kinase (PI3K)/ Beclin-1 pathway plays a role in NDV-induced autophagy and virus production. Collectively, our data provide a unique example of a paramyxovirus that uses autophagy to enhance its production.
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Authors | Chunchun Meng, Zhizhi Zhou, Ke Jiang, Shengqing Yu, Lijun Jia, Yantao Wu, Yanqing Liu, Songshu Meng, Chan Ding |
Journal | Archives of virology
(Arch Virol)
Vol. 157
Issue 6
Pg. 1011-8
(Jun 2012)
ISSN: 1432-8798 [Electronic] Austria |
PMID | 22398914
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- ATG5 protein, human
- Apoptosis Regulatory Proteins
- Autophagy-Related Protein 5
- BECN1 protein, human
- Beclin-1
- Membrane Proteins
- Microtubule-Associated Proteins
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Topics |
- Animals
- Apoptosis Regulatory Proteins
(genetics, metabolism)
- Autophagy
- Autophagy-Related Protein 5
- Beclin-1
- Cell Line, Tumor
- Glioma
(genetics, physiopathology, therapy, virology)
- Humans
- Membrane Proteins
(genetics, metabolism)
- Microtubule-Associated Proteins
(genetics, metabolism)
- Newcastle disease virus
(genetics, physiology)
- Oncolytic Virotherapy
- Virus Replication
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