[Large-scale in vitro preparation of new gp96 tumor vaccine and analysis of its induction of specific anti-tumor immunoresponses].

Abstract

Heat shock protein gp96 isolated from tumor tissues holds great promise for tumor immunotherapy. However, at present only very limited amount of gp96 protein can be isolated from tumor tissues. Here, we reconstituted the yeast-expressed gp96 (recombinant gp96, rgp96) with B16.F10 melanoma antigens in vitro to prepare new gp96 tumor vaccine on large-scale, and analyzed its induction of specific anti-tumor immunoresponses by ELISPOT, IFN-gamma intracellular staining and cytotoxicity assays. Immunization with rgp96-tumor antigen complexes significantly inhibited B16 tumor growth compared with either rgp96 or tumor antigens alone and led to enhancement of tumor-specific T-cell activities, which was found similar to that of tumor tissue derived gp96. Our results therefore may provide bases for large-scale preparation of the new generation of gp96 tumor vaccines.

• Authors: Xiaoli Yan, Changfei Li, Xiaojun Zhang, Ying Ju, Bao Zhao, Songdong Meng
• Journal: Sheng wu gong cheng xue bao = Chinese journal of biotechnology (Sheng Wu Gong Cheng Xue Bao) Vol. 27 Issue 11 Pg. 1598-605 (Nov 2011) ISSN: 1000-3061 [Print] China
• PMID: 22393714 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Cancer Vaccines
• Heat-Shock Proteins
• Recombinant Fusion Proteins
• vitespin

Topics

• Animals
• Cancer Vaccines (biosynthesis, genetics, immunology, therapeutic use)
• Female
• Heat-Shock Proteins (biosynthesis, genetics, immunology, therapeutic use)
• Melanoma, Experimental (therapy)
• Mice
• Mice, Inbred C57BL
• Neoplasm Transplantation
• Recombinant Fusion Proteins (biosynthesis, genetics, immunology, therapeutic use)
• Skin Neoplasms (therapy)
• Yeasts (genetics, metabolism)