Coptisine exert cardioprotective effect through anti-oxidative and inhibition of RhoA/Rho kinase pathway on isoproterenol-induced myocardial infarction in rats.

Abstract

OBJECTIVE:

Because myocardial infarction is a major cause of morbidity and mortality worldwide, protecting the heart from the ischemia is the focus of intense research. Coptisine is an isoquinoline alkaloid extracted form Coptidis Rhizoma. This study aims to elucidate if coptisine is responsible for cardioprotection using myocardial infarction (MI) rat models and investigate its potential mechanism of action.

METHODS:

Myocardial infarction was produced in rats with 85 mgkg(-1) isoproterenol administered subcutaneously twice at an interval of 24 h. The rats were randomized into 7 groups: (I) Normal; (II) ISO; (III) ISO+fasudil; (IV) ISO+isosorbide dinitrate (ISDN) and (V-VII) ISO+coptisine (25, 50 and 100 mgkg(-1)). Cardiac function and markers of cardiac ischemic were assessed after MI.

RESULTS:

Rats pretreated with coptisine (25, 50 and 100 mgkg(-1)) for 21 days and received subcutaneously injected with ISO (85 mgkg(-1)) on the 20th and 21st day at an interval of 24 h. The results suggested that coptisine has strong antioxidant activity, and it can maintain cell membrane integrity, ameliorate mitochondrial respiratory dysfunction, reduce myocardial cells apoptosis, inhibit RhoA/ROCK expression induced by high-dose isoproterenol administration.

CONCLUSIONS:

Coptisine provided cardioprotection in a model of myocardial infarction, and therefore should be considered as a novel adjunctive therapy for attenuating myocardial damage.

• Authors: Li-Li Gong, Lian-Hua Fang, Su-Bo Wang, Jia-Lin Sun, Hai-Lin Qin, Xiao-Xiu Li, Shou-Bao Wang, Guan-Hua Du
• Journal: Atherosclerosis (Atherosclerosis) Vol. 222 Issue 1 Pg. 50-8 (May 2012) ISSN: 1879-1484 [Electronic] Ireland
• PMID: 22387061 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2012 Elsevier Ireland Ltd. All rights reserved.

Chemical References

• Antioxidants
• Cardiotonic Agents
• coptisine
• Berberine
• 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine
• rho-Associated Kinases
• rhoA GTP-Binding Protein
• Isoproterenol
• fasudil

Topics

• 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine (analogs & derivatives, pharmacology)
• Animals
• Antioxidants (pharmacology)
• Apoptosis (drug effects)
• Berberine (analogs & derivatives, therapeutic use)
• Cardiotonic Agents (therapeutic use)
• Isoproterenol
• Male
• Mitochondria, Heart (drug effects)
• Myocardial Infarction (chemically induced, prevention & control)
• Rats
• Rats, Sprague-Dawley
• rho-Associated Kinases (antagonists & inhibitors)
• rhoA GTP-Binding Protein (antagonists & inhibitors)