Neuropilin-2 expression in cancer.

Abstract

AIMS:

Neuropilin-2 is a coreceptor for vascular endothelial growth factor family members. Blockade of neuropilin-2 is able to suppress lymphogenous metastasis in preclinical models. The aim of this study was to validate a protocol for the evaluation of neuropilin-2 protein expression in situ, by comparison with in-situ hybridization, western blotting, and mRNA expression levels.

METHODS AND RESULTS:

Immunohistochemistry was performed on normal human tissues, and whole sections for 79 primary non-small-cell lung carcinomas, 65 primary breast carcinomas, 79 primary colorectal cancers, and 52 metastases. Neuropilin-2 expression was observed in lymphatic and blood vessels from all normal and malignant tissues examined. In addition, 32% of primary non-small-cell lung carcinomas, 15% of primary breast carcinomas and 22% of primary colorectal cancers showed tumour cell expression. Fifty-five primary and nine secondary malignant melanomas were also examined for neuropilin-2 expression by in-situ hybridization. All showed vascular expression, and 85% of primary malignant melanomas showed tumour cell expression.

CONCLUSIONS:

In the majority of lung, breast and colorectal cancers, the effects of anti-neuropilin-2 are likely to be restricted to the vasculature. These results will assist in pharmacokinetic evaluations, tolerability assessments and the choice of setting to evaluate the activity of anti-neuropilin-2 therapies.

• Authors: Adrian M Jubb, Susan M Sa, Navneet Ratti, Laura A Strickland, Maike Schmidt, Christopher A Callahan, Hartmut Koeppen
• Journal: Histopathology (Histopathology) Vol. 61 Issue 3 Pg. 340-9 (Sep 2012) ISSN: 1365-2559 [Electronic] England
• PMID: 22384800 (Publication Type: Journal Article)
• Copyright: © 2012 Blackwell Publishing Ltd.

Chemical References

• Antibodies
• Biomarkers, Tumor
• Neuropilin-2

Topics

• Animals
• Antibodies
• Antibody Specificity
• Biomarkers, Tumor (analysis)
• Blotting, Western
• Humans
• In Situ Hybridization
• Mice
• Neoplasms (metabolism)
• Neuropilin-2 (analysis, metabolism)
• Tissue Array Analysis
• Transcriptome