Nanopatterned cadmium selenide Langmuir-Blodgett platform for leukemia detection.

Abstract

We present results of the studies relating to preparation of Langmuir-Blodgett (LB) monolayers of tri-n-octylphosphine oxide-capped cadmium selenide quantum dots (QCdSe) onto indium-tin oxide (ITO) coated glass substrate. The monolayer behavior has been studied at the air-water interface under various subphase conditions. This nanopatterned platform has been explored to fabricate an electrochemical DNA biosensor for detection of chronic myelogenous leukemia (CML) by covalently immobilizing the thiol-terminated oligonucleotide probe sequence via a displacement reaction. The results of electrochemical response studies reveal that this biosensor can detect target DNA in the range of 10(-6) to 10(-14) M within 120 s, has a shelf life of 2 months, and can be used about 8 times. Further, this nucleic acid sensor has been found to distinguish the CML-positive and the control negative clinical patient samples.

• Authors: Aditya Sharma, Chandra M Pandey, Zimple Matharu, Udit Soni, Sameer Sapra, Gajjala Sumana, Manoj K Pandey, Tathagat Chatterjee, Bansi D Malhotra
• Journal: Analytical chemistry (Anal Chem) Vol. 84 Issue 7 Pg. 3082-9 (Apr 03 2012) ISSN: 1520-6882 [Electronic] United States
• PMID: 22380657 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Cadmium Compounds
• DNA Probes
• Organophosphorus Compounds
• Selenium Compounds
• Tin Compounds
• indium tin oxide
• trioctyl phosphine oxide
• cadmium selenide

Topics

• Base Sequence
• Biosensing Techniques (instrumentation, methods)
• Cadmium Compounds (chemistry)
• DNA Probes (chemistry, genetics)
• Electrochemistry
• Electrodes
• Equipment Reuse
• Humans
• Leukemia, Myelogenous, Chronic, BCR-ABL Positive (diagnosis)
• Nanotechnology (instrumentation, methods)
• Nucleic Acid Hybridization
• Organophosphorus Compounds (chemistry)
• Quantum Dots
• Selenium Compounds (chemistry)
• Surface Properties
• Tin Compounds (chemistry)