Anticonvulsant and antioxidant effects of cyano-carvone and its action on acetylcholinesterase activity in mice hippocampus.

The anticonvulsant effect of cyano-carvone, a monoterpene monocyclic, was investigated in epilepsy model induced by pilocarpine. Cyano-carvone at doses of 25, 50 or 75 mg/kg promoted a reduction of 16.7, 33 and 66.7%, respectively, against pilocarpine-induced seizures, and it was efficacious in increasing both the latency to first seizures and the survival percentage, resulting in 33.3, 67 and 91.7% of protection against death induced by seizures, respectively (P < 0.05). The reference drug atropine (25 mg/kg) also produced a significant protection (100%). Its monoterpene, at 25, 50 and 75 mg/kg, was also capable to increase the latency for installation of status epilepticus induced by pilocarpine, and presented a significant protection against lipid peroxidation and nitrite formation in mice hippocampus (P < 0.05). In addition, it was observed that the cyano-carvone pretreatment increased the acetylcholinesterase activity in mice hippocampus after pilocarpine-induced seizures. The present results clearly indicate the anticonvulsant ability of cyano-carvone, which can be, at least in part, explained by the increased activity of the acetylcholinesterase enzyme. Our data suggest that the action mechanism can also be due to a direct activation of the antioxidant enzymes that could be associated with a reduction observed in oxidative stress in mice hippocampus, probably involving an inhibition of free radical production.
AuthorsDayane Alves Costa, Guilherme Antônio Lopes de Oliveira, Tamires Cardoso Lima, Pauline Sousa dos Santos, Damião Pergentino de Sousa, Rivelilson Mendes de Freitas
JournalCellular and molecular neurobiology (Cell Mol Neurobiol) Vol. 32 Issue 4 Pg. 633-40 (May 2012) ISSN: 1573-6830 [Electronic] United States
PMID22373695 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Anticonvulsants
  • Antioxidants
  • Monoterpenes
  • Acetylcholinesterase
  • Acetylcholinesterase (metabolism)
  • Animals
  • Anticonvulsants (pharmacology, therapeutic use)
  • Antioxidants (pharmacology, therapeutic use)
  • Disease Models, Animal
  • Enzyme Activation (drug effects)
  • Male
  • Mice
  • Monoterpenes (pharmacology, therapeutic use)
  • Oxidative Stress (drug effects, physiology)
  • Status Epilepticus (drug therapy, enzymology, metabolism)

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