Abstract |
The purpose of this study was to investigate the effect of Raf kinase inhibitor protein (RKIP) on the growth, proliferation, invasion and metastasis of triple-negative breast cancer (TNBC) cells to provide experimental evidence for developing future therapies against human TNBC. The pcDNA3.1-RKIP eukaryotic expression vector was constructed and transfected into the TNBC cell line MDA-MB-231. The alterations of the biological characteristics of RKIP-transfected MDA-MB-231 cells were analyzed using the following approaches: a growth curve, a 3-(4,5-dimethylthiazol-2-Yl)-2,5-diphenyltetrazolium bromide (MTT) assay, bromodeoxyuridine ( BrdU) staining and a cell migration assay. The effects of the RKIP gene on MMP-1 and MMP-2 expression were also examined. The pcDNA3.1 empty vector-transfected and mock-transfected MDA-MB-231 cells were used as control groups. Compared with the empty vector-transfected and mock-transfected cells, the cell growth of RKIP-transfected MDA-MB-231 cells was significantly reduced. The empty vector-transfected group was not significantly different compared with the mock-transfected MDA-MB-231 cells. The results of the MTT and BrdU assays demonstrated that the proliferation of pcDNA3.1-RKIP-transfected cells was significantly reduced compared to the control cells (P < 0.05). The result of the cell migration assay suggested that the cross-membrane migration rate of the pcDNA3.1-RKIP-transfected cells was significantly lower than that of the control MDA-MB-231 cells (P < 0.05). We also demonstrated that RKIP may inhibit MMP-1 and MMP-2 expression in MDA-MB-231 cells. The RKIP gene may play a role in inhibiting cellular proliferation. The RKIP gene may also have some inhibitory effects on the invasiveness and metastatic capability of human TNBC cells.
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Authors | Chunfang Hao, Sen Wei, Zhongsheng Tong, Shufen Li, Yehui Shi, Xiaorui Wang, Zhi-hua Zhu |
Journal | Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine
(Tumour Biol)
Vol. 33
Issue 4
Pg. 1159-67
(Aug 2012)
ISSN: 1423-0380 [Electronic] Netherlands |
PMID | 22373584
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- PEBP1 protein, human
- Phosphatidylethanolamine Binding Protein
- Receptors, Estrogen
- Receptors, Progesterone
- Receptor, ErbB-2
- Matrix Metalloproteinase 2
- Matrix Metalloproteinase 1
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Topics |
- Blotting, Western
- Breast Neoplasms
(genetics, metabolism, pathology)
- Cell Line, Tumor
- Cell Movement
(genetics)
- Cell Proliferation
- Cell Survival
(genetics)
- Female
- Gene Expression Regulation, Neoplastic
- Humans
- Matrix Metalloproteinase 1
(metabolism)
- Matrix Metalloproteinase 2
(metabolism)
- Phosphatidylethanolamine Binding Protein
(genetics, metabolism)
- Receptor, ErbB-2
(metabolism)
- Receptors, Estrogen
(metabolism)
- Receptors, Progesterone
(metabolism)
- Reverse Transcriptase Polymerase Chain Reaction
- Time Factors
- Transfection
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