Promotion of glycerol utilization using ethanol and 1-propanol in Schizosaccharomyces pombe.

Abstract

The fission yeast Schizosaccharomyces pombe does not grow in media containing glycerol as a sole carbon source but uses glycerol in the presence of ethanol. Ethanol, but not glycerol, triggered upregulation of gld1+ and fbp1+ during glucose starvation even though gld1+ and fbp1+ are essential for growth on glycerol. This upregulation occurred at a very low concentration of ethanol. The transcriptional regulation of gld1+ was tested in the presence of various alcohols, and both ethanol and 1-propanol were found to induce gld1+ and to support growth in glycerol-containing media. We suggest that S. pombe has a novel ethanol and/or 1-propanol recognition mechanism that upregulates glycerol utilization during glucose starvation.

• Authors: Tomohiko Matsuzawa, Futoshi Hara, Hideki Tohda, Hiroshi Uemura, Kaoru Takegawa
• Journal: Applied microbiology and biotechnology (Appl Microbiol Biotechnol) Vol. 95 Issue 2 Pg. 441-9 (Jul 2012) ISSN: 1432-0614 [Electronic] Germany
• PMID: 22370953 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Culture Media
• Ethanol
• Carbon
• 1-Propanol
• Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating)
• Fructose-Bisphosphatase
• Glycerol

Topics

• 1-Propanol (metabolism)
• Carbon (metabolism)
• Culture Media (chemistry)
• Ethanol (metabolism)
• Fructose-Bisphosphatase (biosynthesis)
• Gene Expression
• Gene Expression Regulation, Fungal
• Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating) (biosynthesis)
• Glycerol (metabolism)
• Schizosaccharomyces (genetics, growth & development, metabolism)
• Transcription, Genetic
• Up-Regulation