Safety and skin delayed-type hypersensitivity response in vervet monkeys immunized with Leishmania donovani sonicate antigen delivered with adjuvants.
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| Abstract |
In this study, we report on the safety and skin delayed-type hypersensitivity (DTH), responses of the Leishmania donovani whole cell sonicate antigen delivered in conjunction with alum-BCG (AlBCG), Montanide ISA 720 (MISA) or Monophosphoryl lipid A (MPLA) in groups of vervet monkeys. Following three intradermal injections of the inoculums on days 0, 28 and 42, safety and DTH responses were assessed. Preliminary tumor necrosis factor alpha (TNF-α) and interferon gamma (IFN-γ) levels were also measured and these were compared with DTH. Only those animals immunized with alum-BCG reacted adversely to the inoculum by producing ulcerative erythematous skin indurations. Non-parametric analysis of variance followed by a post-test showed significantly higher DTH responses in the MISA+Ag group compared with other immunized groups (p < 0.001). The MPLA+Ag group indicated significantly lower DTH responses to the sonicate antigen compared with the AlBCG+Ag group. There was a significant correlation between the DTH and cytokine responses (p < 0.0001). Based on this study we conclude that Leishmania donovani sonicate antigen containing MISA 720 is safe and is associated with a strong DTH reaction following immunization.
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| Authors | Joshua M Mutiso, John C Macharia, Evans Taracha, Kellern Wafula, Hitler Rikoi, Michael M Gicheru |
| Journal | Revista do Instituto de Medicina Tropical de Sao Paulo (Rev Inst Med Trop Sao Paulo) 2012 Jan-Feb Vol. 54 Issue 1 Pg. 37-41 ISSN: 1678-9946 [Electronic] Brazil |
| PMID | 22370752 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't) |
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