Pachyman treatment improves CD4+CD25+ Treg counts and serum interleukin 4 and interferon γ levels in a mouse model of Kawasaki disease.

Abstract

The aim of this study was to explore the effect of pachyman, a mushroom extract, on CD4+CD25+ regulatory T cells (Tregs), serum interleukin 4 (IL-4) and interferon γ (IFN-γ) levels in a mouse model of Kawasaki disease. Lactobacillus casei cell wall extract was diluted to 1 mg/ml in PBS and administered to mice by intraperitoneal injection to establish a model of Kawasaki disease. Sixty female mice were used in this study, 40 of which were randomly assigned to a model (normal saline by gavage, n=20) or experimental group (200 mg/kg/day pachyman by gavage, n=20). The remaining 20 mice were disease and treatment-free, and were used as the control group. Compared to the control mice, mice in the model group exhibited a significantly lower percentage of CD4+CD25+ Tregs and significantly higher serum IL-4 and IFN-γ levels (P<0.05). However, CD4+CD25+ Tregs significantly increased and IL-4 and IFN-γ levels significantly decreased in experimental mice following pachyman treatment (P<0.05). Further analysis showed a negative correlation between CD4+CD25+ Tregs and IL-4/IFN-γ levels (P<0.05). In conclusion, pachyman improves immune function in a mouse model of Kawasaki disease by upregulating CD4+CD25+ Tregs, which may inhibit the cytokine secretion of Th1 and Th2 cells.

• Authors: Mao-Ping Chu, Dan Wang, Ying-Ying Zhang, Bao-Qing Li, Ai-Hua Zhou, Xi-Wen Chen, Yan Qian
• Journal: Molecular medicine reports (Mol Med Rep) Vol. 5 Issue 5 Pg. 1237-40 (May 2012) ISSN: 1791-3004 [Electronic] Greece
• PMID: 22367425 (Publication Type: Journal Article)

Chemical References

• Complex Mixtures
• Glucans
• Interleukin-4
• Interferon-gamma
• pachyman

Topics

• Animals
• CD4 Lymphocyte Count
• Cell Wall (chemistry)
• Complex Mixtures (chemistry, toxicity)
• Disease Models, Animal
• Female
• Glucans (pharmacology)
• Humans
• Interferon-gamma (blood)
• Interleukin-4 (blood)
• Lacticaseibacillus casei (chemistry)
• Mice
• Mice, Inbred BALB C
• Mucocutaneous Lymph Node Syndrome (blood, chemically induced, drug therapy)
• T-Lymphocytes, Regulatory (metabolism)
• Th1 Cells (metabolism)
• Th2 Cells (metabolism)