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Confocal Raman microspectroscopic study of folate receptor-targeted delivery of 6-mercaptopurine-embedded gold nanoparticles in a single cell.

Abstract
We investigate the cellular uptake behaviors and efficacy of folate-coated gold nanoparticles (AuNPs) for the targeted drug delivery system in human cancer cells. Folate-conjugated AuNPs embedded with a purine analogue cancer drug of 6-mercaptopurine (6MP) were assembled via a 1-ethyl-3-[3-dimethylaminopropyl] carbodiimide hydrochloride (EDC) coupling reaction between the amino group of 4-aminobenzenethiol (ABT) and the carboxyl group of folic acid. The assembly of folate and 6MP on AuNPs has been examined by absorption spectroscopy, transmission electron microscopy (TEM), and confocal Raman spectroscopy. The internalization of the conjugated AuNPs inside the folate receptor-positive HeLa and KB cells was checked by TEM and dark-field microscopy (DFM) combined with label-free confocal spectroscopy over the depth variable z at a micrometer resolution. DFM live cell imaging of folate-conjugated AuNPs in HeLa cells indicated that the targeted AuNPs appeared to attach on the cell surfaces and enter into the cell with an hour. The cell viability was also compared to estimate the efficacy of folate-conjugated AuNP delivery systems. Folate receptor-targeted AuNP systems appeared to decrease cancer cell viability both in vitro and in vivo more than did the use of the 6MP-coated AuNPs drug without any targeting systems.
AuthorsJin Park, Won Il Jeon, So Yeong Lee, Kwang-Su Ock, Ji Hye Seo, Jinho Park, Erdene-Ochir Ganbold, Keunchang Cho, Nam Woong Song, Sang-Woo Joo
JournalJournal of biomedical materials research. Part A (J Biomed Mater Res A) Vol. 100 Issue 5 Pg. 1221-8 (May 2012) ISSN: 1552-4965 [Electronic] United States
PMID22359274 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright © 2012 Wiley Periodicals, Inc.
Chemical References
  • Folate Receptors, GPI-Anchored
  • Gold
  • Folic Acid
  • Mercaptopurine
Topics
  • Absorption (drug effects)
  • Animals
  • Cell Survival (drug effects)
  • Drug Delivery Systems (methods)
  • Folate Receptors, GPI-Anchored (metabolism)
  • Folic Acid (metabolism)
  • Gold (chemistry)
  • HeLa Cells
  • Humans
  • Male
  • Mercaptopurine (pharmacology)
  • Metal Nanoparticles (chemistry, ultrastructure)
  • Mice
  • Mice, Inbred BALB C
  • Mice, Nude
  • Spectrum Analysis, Raman (methods)

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