3-O-acetyloleanolic acid induces apoptosis in human colon carcinoma HCT-116 cells.

Abstract

The cytotoxic effect of 3-O-acetyloleanolic acid, an oleanolic acid derivative isolated from the seeds of Vigna sinensis K., was investigated in human colon carcinoma HCT-116 cells. 3-O-acetyloleanolic acid dose-dependently inhibited the viability of HCT-116 cells. Apoptosis was characterized by detection of cell surface annexin V and sub-G1 apoptotic cell populations. The number of immunostained cells with annexin V-FITC was increased after treatment with 3-O-acetyloleanolic acid. The sub-G1 cell population was also increased. Expression of TRAIL-mediated apoptosis signaling-related death receptor DR5 was increased in 3-O-acetyloleanolic acid-treated HCT-116 cells. Activation of caspase-8 and caspase-3, critical mediators of extrinsic apoptosis signaling, was also increased by 3-O-acetyloleanolic acid. The results indicate that 3-O-acetyloleanolic acid induces apoptosis in HCT-116 cells mediated by an extrinsic apoptosis signaling cascade via up-regulation of DR5.

• Authors: Ki Hyun Yoo, Jong-Hwa Park, En Ji Cui, Kyung Il Kim, Joo Yun Kim, Jiyoung Kim, Seong Gil Hong, Nam In Baek, In Sik Chung
• Journal: Phytotherapy research : PTR (Phytother Res) Vol. 26 Issue 10 Pg. 1541-6 (Oct 2012) ISSN: 1099-1573 [Electronic] England
• PMID: 22359244 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2012 John Wiley & Sons, Ltd.

Chemical References

• Receptors, TNF-Related Apoptosis-Inducing Ligand
• Triterpenes
• oleanolic acid 3-acetate
• CASP3 protein, human
• CASP8 protein, human
• Caspase 3
• Caspase 8

Topics

• Apoptosis (drug effects)
• Caspase 3 (metabolism)
• Caspase 8 (metabolism)
• Fabaceae (chemistry)
• HCT116 Cells
• Humans
• Receptors, TNF-Related Apoptosis-Inducing Ligand (metabolism)
• Seeds (chemistry)
• Triterpenes (isolation & purification, pharmacology)
• Up-Regulation