Down-regulation of CRMP-1 in patients with epilepsy and a rat model.

Abstract

The Collapsin Response Mediator Protein-1 (CRMP-1) is a brain specific protein identified as a signaling molecule of Semaphorin-3A and act as axon repellent guidance factor in nervous system. Recent studies indicated that axon guidance molecules may play a role in synaptic reorganization in the adult brain and thereby promote epileptogenesis. This study aimed to investigate expression pattern of CRMP-1 in epileptogenesis. Using double immunofluorescence labeling, immunohistochemistry and western blot analysis, we looked into the CRMP-1 expression in temporal neocortex from patients with temporal lobe epilepsy (TLE) and histological normal temporal neocortex from the controls. We also studied the expression pattern of CRMP-1 in hippocampus and adjacent cortex of a TLE rat model on 6, 24, 72 h, 1, 2 weeks, 1 month, and 2 months post-seizure, and from control rats. CRMP-1 was mainly expressed in the neuronal cytoplasm in the temporal lobe of intractable TLE patients, which was co-expressed with -2. CRMP-1 expression was downregulated in temporal neocortical of TLE patients. In addition, in pilocarpine-induced animal model of epilepsy, CRMP-1 dynamically decreased in a range of 2 months. Thus, our results indicate that CRMP-1 may be involved in the development of TLE.

• Authors: Jing Luo, Kebin Zeng, Chengqu Zhang, Min Fang, Xiaogang Zhang, Qiong Zhu, Liang Wang, Wei Wang, Xuefeng Wang, Guojun Chen
• Journal: Neurochemical research (Neurochem Res) Vol. 37 Issue 7 Pg. 1381-91 (Jul 2012) ISSN: 1573-6903 [Electronic] United States
• PMID: 22359051 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• CRMP1 protein, human
• Nerve Tissue Proteins

Topics

• Adolescent
• Adult
• Animals
• Disease Models, Animal
• Down-Regulation
• Epilepsy (physiopathology)
• Female
• Humans
• Male
• Middle Aged
• Nerve Tissue Proteins (physiology)
• Rats
• Rats, Sprague-Dawley
• Young Adult