Algorithm proposal based on the C. Diff Quik Chek Complete ICT device for detecting Clostridium difficile infection.

Abstract

OBJECTIVE:

To assess a new immunochromatography (ICT) test that detects glutamate dehydrogenase (GDH) antigen and Clostridium difficile toxin A/B simultaneously, and to propose an algorithm for the diagnosis of C. difficile infection (CDI) based on this test.

METHODS:

We analysed 970 stool samples. Discrepant results between GDH and toxin A/B were resolved using toxigenic culture as the reference.

RESULTS:

This test enabled us to obtain a conclusive result in <30min in 93.8% of the samples. Among the discrepant results (GDH (+)/Toxin A/B (-)), 41.7% (25/60) were found to be toxigenic C. difficile by toxigenic culture.

CONCLUSION:

This test has a high sensitivity and specificity for the diagnosis of CDI.

• Authors: M A Angeles Orellana-Miguel, Adela Alcolea-Medina, Laura Barrado-Blanco, Joaquín Rodriguez-Otero, Fernando Chaves-Sánchez
• Journal: Enfermedades infecciosas y microbiologia clinica (Enferm Infecc Microbiol Clin) Vol. 31 Issue 2 Pg. 97-9 (Feb 2013) ISSN: 1578-1852 [Electronic] Spain
• PMID: 22341752 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2011 Elsevier España, S.L. All rights reserved.

Chemical References

• Antigens, Bacterial
• Azure Stains
• Diff Quik
• Xanthenes
• Glutamate Dehydrogenase
• Methylene Blue

Topics

• Algorithms
• Antigens, Bacterial (analysis)
• Azure Stains
• Chromatography, Affinity (instrumentation)
• Clostridium Infections (diagnosis)
• Glutamate Dehydrogenase (immunology)
• Humans
• Methylene Blue
• Xanthenes