Effects of N1-methylnicotinamide on oxidative and glycooxidative stress markers in rats with streptozotocin-induced diabetes mellitus.

Abstract

OBJECTIVES:

This study was focused on the monitoring how the anti-inflammatory substance, N(1)-methylnicotinamide (MNA), could influence oxidation and glycooxidation stress markers in rats under conditions of streptozotocin (STZ)-induced diabetes mellitus.

METHODS:

Diabetes mellitus was induced in 60 male Wistar rats by intraperitoneal injection of STZ and after 7 days diabetic animals were allocated to five groups according to the dose of MNA administered for 7 weeks. The degree of DNA damage in lymphocytes, as well as advanced glycation endproducts (AGEs), protein carbonyls, lipid peroxides, and total antioxidant capacity (TEAC) in plasma were measured.

RESULTS:

Glycation damage to proteins (represented by AGEs level) was significantly increased in all diabetic groups compared to untreated non-diabetic animals. MNA did not affect TEAC of plasma in any group of diabetic rats. Supplementation of diabetic rats with MNA at the dose of 200 mg/kg resulted in decreased protein carbonyls (from 0.0818±0.0091 to 0.0558±0.0044 nmol/mg proteins; P<0.05, n=15) and DNA oxidation, reflected by the levels of 8-oxoG (0.6302±0.085 vs. 0.9213±0.108 8-oxoG/10(6) G; P<0.05, n=15), compared to untreated diabetic animals.

DISCUSSION:

Our results demonstrated that MNA at suitable concentrations could influence oxidative modifications of proteins and DNA.

• Authors: Zuzana Országhová, Ol'ga Uličná, Anna Liptáková, Ingrid Zitňanová, Jana Muchová, Cezary Watala, Zdeňka Duračková
• Journal: Redox report : communications in free radical research (Redox Rep) Vol. 17 Issue 1 Pg. 1-7 ( 2012) ISSN: 1743-2928 [Electronic] England
• PMID: 22340509 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antioxidants
• Biomarkers
• Glycation End Products, Advanced
• Lipid Peroxides
• Niacinamide
• Streptozocin
• N(1)-methylnicotinamide

Topics

• Animals
• Antioxidants (metabolism)
• Biomarkers (analysis)
• DNA Damage
• Diabetes Mellitus, Experimental (metabolism, pathology)
• Drug Evaluation, Preclinical
• Glycation End Products, Advanced (analysis)
• Lipid Peroxidation (drug effects)
• Lipid Peroxides (blood)
• Lymphocytes (metabolism, pathology)
• Male
• Niacinamide (analogs & derivatives, pharmacology)
• Oxidation-Reduction
• Oxidative Stress
• Protein Carbonylation (drug effects)
• Rats
• Rats, Wistar
• Streptozocin