Abstract | OBJECTIVES: METHODS: Monocultures of human meningioma cells and meningioma-primary human macrophage co-cultures were infected with meningococci (10(2)-10(8) cfu/monolayer) or treated with isolated outer membranes or purified LPS (0.1-100 ng/monolayer) from N. meningitidis. CyP (1-20 μg/monolayer) was added at intervals from t = 0 to 4 h, with and without benzylpenicillin (1-20 μg/monolayer). The antagonistic effect of CyP and its adjunctive properties to benzylpenicillin administration was determined by measuring cytokine levels in culture supernatants after 24 h. RESULTS: CyP significantly inhibited (P < 0.05) the secretion of interleukin (IL)-6, IL-8, monocyte chemoattractant protein (MCP)-1 and RANTES ('regulated upon activation, normal T cell expressed and secreted') (overall reduction levels from 50% to >95%) by meningioma cell lines and meningioma-macrophage co-cultures challenged with either live meningococci or bacterial components. Inhibition was effective when CyP was added within 2 h of challenge (P < 0.05) and was still pronounced by 4 h. In the co-culture model, CyP alone partially inhibited IL-1β secretion, but did not prevent tumour necrosis factor (TNF)-α secretion, whereas penicillin alone inhibited IL-1β and TNF-α but conversely did not reduce MCP-1 and RANTES secretion. However, coadministration of CyP and penicillin in both models had an additive effect and restored the overall inhibitory profile. CONCLUSIONS:
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Authors | Rebecca Oliver, Karl J Staples, John Heckels, Carlo Rossetti, Monica Molteni, Myron Christodoulides |
Journal | The Journal of antimicrobial chemotherapy
(J Antimicrob Chemother)
Vol. 67
Issue 5
Pg. 1145-54
(May 2012)
ISSN: 1460-2091 [Electronic] England |
PMID | 22334603
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Anti-Bacterial Agents
- Cytokines
- Immunologic Factors
- Lipopolysaccharides
- Penicillin G
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Topics |
- Anti-Bacterial Agents
(pharmacology)
- Cells, Cultured
- Coculture Techniques
- Cytokines
(metabolism)
- Epithelial Cells
(immunology)
- Humans
- Immunologic Factors
(pharmacology)
- Lipopolysaccharides
(antagonists & inhibitors)
- Macrophages
(immunology)
- Meningitis, Meningococcal
(immunology, physiopathology)
- Neisseria meningitidis
(immunology, pathogenicity)
- Penicillin G
(pharmacology)
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