Abstract | OBJECTIVE: METHODS: Stool samples were collected from 60 patients with colorectal cancer, 17 patients with adenoma and 30 normal volunteers. And fecal DNA was extracted and vimentin, OMSR and TFPI2 gene methylation status was analyzed by methylation-specific polymerase chain reaction (MSP). RESULTS: The methylated vimentin, OMSR and TFPI2 was detected in 53.3% (32/60), 68.3% (41/60) and 75.0% (45/60) of colorectal cancer, and 5, 7 and 11 cases of colorectal adenoma respectively. The sensitivities of combined study, using three markers for the detection of colorectal cancer and colorectal adenomas, were 86.7% (26/30) and 76.5% (13/17) respectively. And the specificity was 86.7% (52/60). CONCLUSION: As a feasible epigenetic marker, promoter hypermethylation for vimentin, OMSR and TFPI2 in stool samples is a sensitive, specific and noninvasive alternative for colorectal cancer screening.
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Authors | Jin-Ping Zhang, Jing Wang, Yin-Li Gui, Qiong-Qiong Zhu, Zhi-Wei Xu, Jian-Sheng Li |
Journal | Zhonghua yi xue za zhi
(Zhonghua Yi Xue Za Zhi)
Vol. 91
Issue 35
Pg. 2482-4
(Sep 20 2011)
ISSN: 0376-2491 [Print] China |
PMID | 22321844
(Publication Type: Journal Article)
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Chemical References |
- Biomarkers, Tumor
- Receptors, Oncostatin M
- Vimentin
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Topics |
- Biomarkers, Tumor
(metabolism)
- Colorectal Neoplasms
- DNA Methylation
- Feces
- Humans
- Receptors, Oncostatin M
- Vimentin
(metabolism)
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