Impaired autophagy and organellar dysfunction in pancreatitis.

Abstract

Recent findings from our group, obtained on experimental in vivo and ex vivo models of pancreatitis, reveal that this disease causes a profound dysfunction of key cellular organelles, lysosomes and mitochondria. We found that autophagy, the main cellular degradative, lysosome-driven process, is activated but also impaired in acute pancreatitis because of its' inefficient progression/resolution (flux) resulting from defective function of lysosomes. One mechanism underlying the lysosomal dysfunction in pancreatitis is abnormal processing (maturation) and activation of cathepsins, major lysosomal hydrolases; another is a decrease in pancreatic levels of key lysosomal membrane proteins LAMP-1 and LAMP-2. Our data indicate that lysosomal dysfunction plays an important initiating role in pancreatitis pathobiology. The impaired autophagy mediates vacuole accumulation in acinar cells; furthermore, the abnormal maturation and activation of cathepsins leads to increase in intra-acinar trypsin, the hallmark of pancreatitis; and LAMP-2 deficiency causes inflammation and acinar cell necrosis. Thus, the autophagic and lysosomal dysfunctions mediate key pathologic responses of pancreatitis. On the other hand, we showed that pancreatitis causes acinar cell mitochondria depolarization, mediated by the permeability transition pore (PTP). Genetic (via deletion of cyclophilin D) inactivation of PTP prevents mitochondrial depolarization and greatly ameliorates the pathologic responses of pancreatitis. Further, our data suggest that mitochondrial damage, by stimulating autophagy, increases the demand for efficient lysosomal degradation and therefore aggravates the pathologic consequences of lysosomal dysfunction. Thus, the combined autophagic, lysosomal and mitochondrial dysfunctions are key to the pathogenesis of pancreatitis.

• Authors: Ilya Gukovsky, Stephen J Pandol, Olga A Mareninova, Natalia Shalbueva, Wenzhuo Jia, Anna S Gukovskaya
• Journal: Journal of gastroenterology and hepatology (J Gastroenterol Hepatol) Vol. 27 Suppl 2 Pg. 27-32 (Mar 2012) ISSN: 1440-1746 [Electronic] Australia
• PMID: 22320913 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, U.S. Gov't, Non-P.H.S., Review)
• Copyright: © 2012 Journal of Gastroenterology and Hepatology Foundation and Blackwell Publishing Asia Pty Ltd.

Chemical References

• Lysosome-Associated Membrane Glycoproteins
• Cathepsins

Topics

• Animals
• Autophagy
• Cathepsins (metabolism)
• Humans
• Lysosome-Associated Membrane Glycoproteins (metabolism)
• Lysosomes (metabolism, pathology)
• Mitochondria (metabolism, pathology)
• Pancreas (metabolism, pathology, physiopathology)
• Pancreatitis (metabolism, pathology, physiopathology)