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Embryonic neural cell adhesion molecule in cerebrospinal fluid of younger children: age-dependent decrease during the first year.

Abstract
Poly-alpha-2,8-N-acetylneuraminic acid (poly-alpha-2,8-NeuAc) is developmentally expressed in neural tissue of higher animals, where it is covalently attached to the neural cell adhesion molecule (NCAM), a large integral membrane glycoprotein mediating cell-cell adhesion during neuronal development. NCAM exists in several molecular forms, of which only embryonic NCAM carries lengthy chains (n greater than 5) of poly-alpha-2,8-NeuAc. Chemically identical poly-alpha-2,8-NeuAc of bacterial origin is an important virulence factor in infections caused by Neisseria meningitidis group B and Escherichia coli K1, the predominant pathogens of bacterial meningitis. A quantitative enzyme-linked immunoassay was developed using monoclonal antibody (MAb) 735, an MAb specifically recognizing poly-alpha-2,8-NeuAc, and applied to CSF specimens from younger children. Poly-alpha-2,8-NeuAc contents were within the range of 20-0.2 micrograms/ml, decreasing from day 1 to day 300. Immunoprecipitation, immunoblot with a rabbit anti-mouse NCAM serum recognizing the protein part of human NCAM by cross-reactivity, affinity enrichment using immobilized MAb 735, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed that poly-alpha-2,8-NeuAc in CSF is bound to human NCAM, probably NCAM-120.
AuthorsC Weisgerber, M Husmann, M Frosch, C Rheinheimer, W Peuckert, I Görgen, D Bitter-Suermann
JournalJournal of neurochemistry (J Neurochem) Vol. 55 Issue 6 Pg. 2063-71 (Dec 1990) ISSN: 0022-3042 [Print] England
PMID2230809 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Antigens, Surface
  • Cell Adhesion Molecules, Neuronal
  • Immune Sera
  • Sialic Acids
  • neural cell surface antigen BSP-2
Topics
  • Antigens, Surface (cerebrospinal fluid)
  • Cell Adhesion Molecules, Neuronal (cerebrospinal fluid, immunology)
  • Chromatography, Affinity
  • Electrophoresis, Gel, Two-Dimensional
  • Electrophoresis, Polyacrylamide Gel
  • Embryo, Mammalian (metabolism)
  • Humans
  • Immune Sera (immunology)
  • Immunoblotting
  • Infant
  • Infant, Newborn
  • Precipitin Tests
  • Sialic Acids

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