Proteomic analysis of secretion from human transplanted submandibular gland replacing lacrimal gland with severe keratoconjunctivitis sicca.

Abstract

PURPOSE:

Proteomic analysis of secretions from transplanted or non-transplanted submandibular glands in patients with severe keratoconjunctivitis sicca and tears from normal eyes.

EXPERIMENTAL DESIGN:

Secretions from submandibular glands transplanted to replace lacrimal glands and non-transplanted submandibular glands were collected at 1year from 5 patients with severe keratoconjunctivitis sicca undergoing transplantation, and tears were collected from 3 normal subjects. 2-D electrophoresis (2-DE), then mass spectrometry was used to identify proteins. Western blot analysis was used to confirm protein expression.

RESULTS:

We identified 34 and 11 distinct proteins in the saliva from transplanted submandibular glands and tears, respectively. The saliva from transplanted submandibular glands contained almost all the proteins abundant in tear fluid. The functions of identified proteins in the saliva from transplanted submandibular gland were mainly immune response and anti-bacterial. In total, 7 proteins showed differential expression between the saliva of transplanted and non-transplanted submandibular glands. The upregulation of short palate, lung and nasal epithelium carcinoma-associated protein 2 and carbonic anhydrase VI was confirmed by Western blot analysis.

CONCLUSIONS:

Identified proteins in saliva from transplanted submandibular glands may protect ocular structures. These findings can help in understanding the functional status of transplanted submandibular glands.

• Authors: Bing Song, Lei Zhang, Xiao-jing Liu, Chong Ding, Li-ling Wu, Ye-hua Gan, Guang-yan Yu
• Journal: Biochimica et biophysica acta (Biochim Biophys Acta) Vol. 1824 Issue 4 Pg. 550-60 (Apr 2012) ISSN: 0006-3002 [Print] Netherlands
• PMID: 22306964 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2012 Elsevier B.V. All rights reserved.

Chemical References

• BPIFA2 protein, human
• Proteome
• Salivary Proteins and Peptides
• Carbonic Anhydrases
• carbonic anhydrase VI

Topics

• Adult
• Carbonic Anhydrases (genetics, metabolism)
• Case-Control Studies
• Electrophoresis, Gel, Two-Dimensional
• Female
• Humans
• Keratoconjunctivitis Sicca (metabolism, therapy)
• Lacrimal Apparatus (surgery)
• Male
• Middle Aged
• Proteome (genetics, metabolism)
• Proteomics
• Saliva (metabolism)
• Salivary Proteins and Peptides (genetics, metabolism)
• Submandibular Gland (metabolism, transplantation)
• Tears (metabolism)
• Up-Regulation
• Young Adult