Reactive oxygen species from chloroplasts contribute to 3-acetyl-5-isopropyltetramic acid-induced leaf necrosis of Arabidopsis thaliana.

Abstract

3-Acetyl-5-isopropyltetramic acid (3-AIPTA), a derivate of tetramic acid, is responsible for brown leaf-spot disease in many plants and often kills seedlings of both mono- and dicotyledonous plants. To further elucidate the mode of action of 3-AIPTA, during 3-AIPTA-induced cell necrosis, a series of experiments were performed to assess the role of reactive oxygen species (ROS) in this process. When Arabidopsis thaliana leaves were incubated with 3-AIPTA, photosystem II (PSII) electron transport beyond Q(A) (the primary plastoquinone acceptor of PSII) and the reduction of the end acceptors at the PSI acceptor side were inhibited; this was followed by increase in charge recombination and electron leakage to O(2), resulting in chloroplast-derived oxidative burst. Furthermore, the main antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX) lost their activity. Excess ROS molecules directly attacked a variety of cellular components and subsequently caused electrolyte leakage, lipid peroxidation and cell membrane disruption. Finally, this led to cell destruction and leaf tissue necrosis. Thus, 3-AIPTA-triggered leaf necrosis of Arabidopsis was found to be a result of direct oxidative injury from the chloroplast-originated ROS burst initiated by the inhibition of normal photosynthetic electron transport.

• Authors: Shiguo Chen, Chunyan Yin, Reto Jörg Strasser, Govindjee, Chunlong Yang, Sheng Qiang
• Journal: Plant physiology and biochemistry : PPB (Plant Physiol Biochem) Vol. 52 Pg. 38-51 (Mar 2012) ISSN: 1873-2690 [Electronic] France
• PMID: 22305066 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2011 Elsevier Masson SAS. All rights reserved.

Chemical References

• Antioxidants
• Photosystem II Protein Complex
• Reactive Oxygen Species
• 3-acetyl-5-isopropyltetramic acid
• Tenuazonic Acid
• Hydrogen Peroxide
• Ascorbate Peroxidases
• Catalase
• Superoxide Dismutase

Topics

• Antioxidants (metabolism)
• Arabidopsis (drug effects, enzymology, physiology)
• Ascorbate Peroxidases (drug effects, metabolism)
• Catalase (drug effects, metabolism)
• Cell Membrane (drug effects)
• Cell Survival
• Chloroplasts (drug effects, metabolism)
• Electron Transport (drug effects)
• Hydrogen Peroxide (metabolism)
• Lipid Peroxidation (drug effects)
• Oxidative Stress (drug effects)
• Photosynthesis (drug effects)
• Photosystem II Protein Complex (drug effects)
• Plant Leaves (drug effects, enzymology, metabolism)
• Reactive Oxygen Species (metabolism)
• Seedlings (drug effects, enzymology, metabolism)
• Superoxide Dismutase (drug effects, metabolism)
• Tenuazonic Acid (analogs & derivatives, pharmacology)