A nebulized gelatin nanoparticle-based CpG formulation is effective in immunotherapy of allergic horses.

Abstract

PURPOSE:

In the recent years, nanotechnology has boosted the development of potential drug delivery systems and material engineering on nanoscale basis in order to increase drug specificity and reduce side effects. A potential delivery system for immunostimulating agents such as cytosine-phosphate-guanine-oligodeoxynucleotides (CpG-ODN) needs to be developed to maximize the efficacy of immunotherapy against hypersensitivity. In this study, an aerosol formulation of biodegradable, biocompatible and nontoxic gelatin nanoparticle-bound CpG-ODN 2216 was used to treat equine recurrent airway obstruction in a clinical study.

METHODS:

Bronchoalveolar lavage fluid was obtained from healthy and allergic horses to quantify Th1/Th2 cytokine levels before and after inhalation regimen. Full clinical examinations were performed to evaluate the therapeutic potential of this nebulized gelatin nanoparticle-based CpG formulation.

RESULTS:

Most remarkable was that regulatory anti-inflammatory and anti-allergic cytokine IL-10 expression was significantly triggered by five consecutive inhalations. Thorough assessment of clinical parameters following nanoparticle treatment indicated a partial remission of the allergic condition.

CONCLUSION:

Thus this study, for the first time, showed effectiveness of colloidal nanocarrier-mediated immunotherapy in food-producing animals with potential future applicability to other species including humans.

• Authors: John Klier, Sebastian Fuchs, Anna May, Ulrike Schillinger, Christian Plank, Gerhard Winter, Conrad Coester, Heidrun Gehlen
• Journal: Pharmaceutical research (Pharm Res) Vol. 29 Issue 6 Pg. 1650-7 (Jun 2012) ISSN: 1573-904X [Electronic] United States
• PMID: 22302522 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Adjuvants, Immunologic
• Aerosols
• CpG ODN 2216
• Cytokines
• Drug Carriers
• Oligodeoxyribonucleotides
• Toll-Like Receptor 9
• Gelatin

Topics

• Adjuvants, Immunologic (administration & dosage, chemistry)
• Administration, Inhalation
• Aerosols
• Airway Obstruction (immunology, therapy, veterinary)
• Animals
• Bronchoalveolar Lavage Fluid (immunology)
• Cells, Cultured
• Chemistry, Pharmaceutical
• Cytokines (metabolism)
• Drug Administration Schedule
• Drug Carriers
• Gelatin (chemistry)
• Horse Diseases (immunology, therapy)
• Horses
• Immunotherapy (veterinary)
• Nanoparticles
• Nanotechnology
• Nebulizers and Vaporizers
• Oligodeoxyribonucleotides (administration & dosage, chemistry)
• Recurrence
• Respiratory Hypersensitivity (immunology, therapy, veterinary)
• Technology, Pharmaceutical (methods)
• Th1 Cells (immunology)
• Th2 Cells (immunology)
• Toll-Like Receptor 9 (agonists)