Abstract | BACKGROUND: Various approaches have been evaluated for generation of efficient immune response against tumor antigens. Our approach exploits usage of particulate delivery to generate immune response against prostate cancer antigens. PURPOSE: METHODS: The whole cell lysate (WCL) was obtained from TRAMP C2 murine prostate cancer cell line and was formulated into particles using one step spray drying process. For in vivo studies, 4-6 week old C57BL/6 male mice were vaccinated orally biweekly for 10 weeks. Serum samples were analyzed at regular intervals to determine serum IgG levels. The mice were then challenged with live TRAMP C2 cells to determine efficacy of the vaccine. RESULTS: The serum IgG levels of vaccinated animals were higher compared to that of the controls. Moreover, the tumor growth was retarded significantly in the vaccinated mice compared to that of controls (p < 0.001). CONCLUSIONS:
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Authors | Archana Akalkotkar, Suprita A Tawde, Lipika Chablani, Martin J D'Souza |
Journal | Journal of drug targeting
(J Drug Target)
Vol. 20
Issue 4
Pg. 338-46
(May 2012)
ISSN: 1029-2330 [Electronic] England |
PMID | 22296308
(Publication Type: Journal Article)
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Chemical References |
- Cancer Vaccines
- Drug Carriers
- Immunoglobulin G
- Lectins
- lectin, Aleuria aurantia
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Topics |
- Administration, Oral
- Animals
- Cancer Vaccines
(administration & dosage, immunology, therapeutic use)
- Cell Line, Tumor
- Cell Survival
(drug effects)
- Drug Carriers
(chemistry)
- Drug Screening Assays, Antitumor
- Enzyme-Linked Immunosorbent Assay
- Immunoglobulin G
(blood)
- Lectins
(chemistry)
- Male
- Mice
- Mice, Inbred C57BL
- Microscopy, Electron, Scanning
- Particle Size
- Peyer's Patches
(drug effects, immunology)
- Prostatic Neoplasms
(immunology, prevention & control)
- Surface Properties
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