Abstract | BACKGROUND AND OBJECTIVE: METHODS: Eleven control never-smokers, six control ex-smokers without COPD and 13 COPD patients participated in the lung tissue study. The expression of cholesterol 25-hydroxylase in the lung was investigated. Twelve control subjects and 17 patients with COPD also participated in the sputum study. The concentrations of 25-HC in sputum were quantified by liquid chromatography/mass spectrometry/mass spectrometry analysis. To elucidate the role of 25-HC in neutrophilic inflammation of the airways, the correlation between 25-HC levels and neutrophil counts in sputum was investigated. RESULTS: The expression of cholesterol 25-hydroxylase was significantly enhanced in lung tissue from COPD patients compared with that from control subjects. Cholesterol 25-hydroxylase was localized in alveolar macrophages and pneumocytes of COPD patients. The concentration of 25-HC in sputum was significantly increased in COPD patients and was inversely correlated with percent of predicted forced vital capacity, forced expiratory volume in 1 s and diffusing capacity of carbon monoxide. The concentrations of 25-HC in sputum were significantly correlated with sputum interleukin-8 levels and neutrophil counts. CONCLUSIONS: 25-HC production was enhanced in the airways of COPD patients and may play a role in neutrophilic inflammation.
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Authors | Hisatoshi Sugiura, Akira Koarai, Tomohiro Ichikawa, Yoshiaki Minakata, Kazuto Matsunaga, Tsunahiko Hirano, Keiichiro Akamatsu, Satoru Yanagisawa, Makoto Furusawa, Yumiko Uno, Masashi Yamasaki, Yoshinori Satomi, Masakazu Ichinose |
Journal | Respirology (Carlton, Vic.)
(Respirology)
Vol. 17
Issue 3
Pg. 533-40
(Apr 2012)
ISSN: 1440-1843 [Electronic] Australia |
PMID | 22295989
(Publication Type: Journal Article)
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Copyright | © 2012 The Authors. Respirology © 2012 Asian Pacific Society of Respirology. |
Chemical References |
- CXCL8 protein, human
- Hydroxycholesterols
- Interleukin-8
- 25-hydroxycholesterol
- Steroid Hydroxylases
- cholesterol 25-hydroxylase
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Topics |
- Aged
- Alveolar Epithelial Cells
(chemistry, enzymology)
- Female
- Humans
- Hydroxycholesterols
(analysis, metabolism)
- Interleukin-8
(analysis)
- Leukocyte Count
- Lung
(chemistry, enzymology, physiopathology)
- Macrophages, Alveolar
(chemistry, enzymology)
- Male
- Middle Aged
- Neutrophils
(enzymology)
- Pulmonary Disease, Chronic Obstructive
(enzymology, physiopathology)
- Respiratory Function Tests
- Smoking
(adverse effects)
- Sputum
(chemistry, enzymology)
- Steroid Hydroxylases
(analysis)
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