Abstract |
The present work evaluates the effect of olive oil phenols on NF-κB activity in human gastric adenocarcinoma cells. The total phenol content was measured by the Folin Ciocalteu method, whereas the composition was assessed by LC-MS/MS analysis. Secoiridoids represented 71% and 83% of the Italian and Spanish extracts, respectively, phenol alcohols were in the range 9-13%. Ligustroside aglycone was the most abundant (37% and 46%, respectively, in the Italian and Spanish sample), and the concentration of flavonoids AP and LU was below 1%. Phenol extracts were assayed at 0.25-7.5 µg/mL, whereas single compounds were at 0.5-25 µM. Both the extracts inhibited the NF-κB driven transcription in a concentration-dependent manner: IC(50) for the Italian and the Spanish extract were 0.86 and 1.28 µg/mL, respectively. The IC(50) for individual compounds ranged from 4.5 to 13 µM. All the compounds under study inhibited nuclear translocation as well. The data suggest that consumption of extra-virgin olive oil may be beneficial for preventing the onset of gastric inflammation leading to more serious diseases.
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Authors | Enrico Sangiovanni, Elisa Colombo, Marco Fumagalli, Federico Abbiati, Donatella Caruso, Mario Dell'Agli |
Journal | Phytotherapy research : PTR
(Phytother Res)
Vol. 26
Issue 10
Pg. 1569-71
(Oct 2012)
ISSN: 1099-1573 [Electronic] England |
PMID | 22294468
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2012 John Wiley & Sons, Ltd. |
Chemical References |
- Flavonoids
- Iridoids
- Lignans
- NF-kappa B
- Olive Oil
- Phenols
- Plant Oils
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Topics |
- Cell Line, Tumor
- Chromatography, Liquid
- Flavonoids
(analysis)
- Humans
- Inhibitory Concentration 50
- Iridoids
(analysis)
- Lignans
(analysis)
- NF-kappa B
(antagonists & inhibitors)
- Olive Oil
- Phenols
(pharmacology)
- Plant Oils
(chemistry, pharmacology)
- Tandem Mass Spectrometry
- Transcription, Genetic
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