Abstract | BACKGROUND & AIMS: METHODS: RESULTS: Samples from patients with EoE, GERD, or Barrett's esophagus had similar increases in concentrations of eosinophils. Patients with GERD or EoE did not differ in amount of basal zone hyperplasia, microabscesses, spongiosis, eosinophil distribution, maximum eosinophils/high-power field (HPF), or composite histologic scores. Samples from all 3 groups had high levels of EDN and MBP; the levels of eosinophil products were correlated (ρ = 0.93). Extracellular staining for EDN was greater than intracellular staining (2.67 of 3 vs 1.86 of 3); levels tended to be greater in samples from patients with EoE than GERD (P = .05) or Barrett's esophagus (P = .06). Detection of EDN correlated with peak numbers of eosinophils/HPF (ρ = 0.6 for intracellular and extracellular staining). Peak numbers of tryptase-positive mast cells/HPF were significantly greater in samples from patients with EoE than GERD or Barrett's esophagus (P = .01 and .005, respectively). The Spearman correlation between eosinophil and mast cell density was a ρ value of 0.2. CONCLUSIONS: Biopsy samples from patients with GERD and EoE, matched for esophageal eosinophilia, have similar changes in histology and levels of EDN and MBP, whereas mast cells from patients with EoE have higher levels of these products. The presence of esophageal eosinophils, rather than etiology, could be the most important determinant of epithelial response.
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Authors | Srividya Sridhara, Karthik Ravi, Thomas C Smyrk, Hirohito Kita, Gail M Kephart, Catherine R Weiler, David A Katzka |
Journal | Clinical gastroenterology and hepatology : the official clinical practice journal of the American Gastroenterological Association
(Clin Gastroenterol Hepatol)
Vol. 10
Issue 7
Pg. 735-41
(Jul 2012)
ISSN: 1542-7714 [Electronic] United States |
PMID | 22289868
(Publication Type: Journal Article)
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Copyright | Copyright © 2012 AGA Institute. Published by Elsevier Inc. All rights reserved. |
Chemical References |
- Proteoglycans
- PRG2 protein, human
- Eosinophil-Derived Neurotoxin
- Eosinophil Major Basic Protein
- Tryptases
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Topics |
- Adult
- Aged
- Aged, 80 and over
- Biopsy
- Diagnosis, Differential
- Eosinophil Major Basic Protein
(analysis)
- Eosinophil-Derived Neurotoxin
(analysis)
- Eosinophilic Esophagitis
(diagnosis, pathology)
- Eosinophils
(pathology)
- Female
- Gastroesophageal Reflux
(diagnosis, pathology)
- Humans
- Immunohistochemistry
- Leukocyte Count
- Male
- Mast Cells
(pathology)
- Middle Aged
- Proteoglycans
(analysis)
- Tryptases
(analysis)
- Young Adult
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