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Plant chitinase III Ziz m 1 stimulates multiple cytokines, most predominantly interleukin-13, from peripheral blood mononuclear cells of latex-fruit allergic patients.

AbstractBACKGROUND:
Indian jujube is a fruit abundantly cultivated in Taiwan. Its major allergen in latex-fruit syndrome is Ziz m 1 of the chitinase III family. The Ziz m 1 Pichia (rZiz m 1-P) has chitinase activity but not Ziz m 1 E. coli (rZiz m 1-E).
OBJECTIVE:
This study examined whether plant chitinase III, using rZiz m 1-P and rZiz m 1-E, can stimulate allergic inflammation similar to that of mammalian chitinases.
METHODS:
Five patients allergic to latex-Indian jujube and five nonallergic controls were evaluated. Their peripheral blood mononuclear cells (PBMC) were cultured with rZiz m 1-E or rZiz m 1-P and pulsed with phorbol 12-myristate 13-acetate. Eleven cytokines were measured by FlowCytomix human Th1/Th2 plex kit and interleukin (IL)-13 by sandwich enzyme-linked immunosorbent assay (ELISA).
RESULTS:
Interleukin-13 significantly increased in rZiz m 1-P stimulated PBMC of allergic subjects but was undetectable when stimulated with rZiz m 1-E. The stimulation index significantly increased in IL-13 (380.6 ± 77.33 vs 13.70 ± 6.92), IL-5 (6.70 ± 0.59 vs 0.70 ± 0.37), IL-1β (32.70 ± 0.83 vs 2.10 ± 1.29), and tumor necrosis factor beta (TNF-β) (17.10 ± 2.66 vs 1.50 ± 0.66) between allergic and nonallergic subjects after rZiz m 1-P stimulation. There was no difference in terms of IL-2, IFN-γ, IL-8, and TNF-α production.
CONCLUSIONS:
The biological function of chitinase activity is required for Ziz m 1 to induce a Th2-specific immune response. This is the first report on PBMC responses of latex-fruit syndrome subjects toward an active exogenous plant class III chitinase that can stimulate multiple cytokines, especially IL-13, from allergic subjects. This implies the role of cross-reactive food allergens in propagating allergic inflammation among allergic subjects.
AuthorsMey-Fann Lee, Shyh-Jye Lin, Nancy M Wang, Hsin-Ju Wu, Yi-Hsing Chen
JournalAnnals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology (Ann Allergy Asthma Immunol) Vol. 108 Issue 2 Pg. 113-6 (Feb 2012) ISSN: 1534-4436 [Electronic] United States
PMID22289730 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright © 2012 American College of Allergy, Asthma & Immunology. Published by Elsevier Inc. All rights reserved.
Chemical References
  • Allergens
  • Antigens, Plant
  • Cytokines
  • Interleukin-13
  • Plant Proteins
  • Recombinant Proteins
  • Ziz m 1 protein, Indian jujube
  • 4-O-methyl-12-O-tetradecanoylphorbol 13-acetate
  • chitinase, class III
  • Chitinase
  • Tetradecanoylphorbol Acetate
Topics
  • Adult
  • Allergens (genetics, immunology)
  • Antigens, Plant
  • Cells, Cultured
  • Chitinase (genetics, immunology)
  • Cloning, Molecular
  • Cross Reactions
  • Cytokines (biosynthesis)
  • Escherichia coli (genetics)
  • Female
  • Food Hypersensitivity (immunology)
  • Humans
  • Interleukin-13 (biosynthesis, genetics)
  • Latex Hypersensitivity (immunology)
  • Leukocytes, Mononuclear (immunology)
  • Lymphocyte Activation
  • Male
  • Middle Aged
  • Pichia (genetics)
  • Plant Proteins (genetics, immunology)
  • Recombinant Proteins (genetics, immunology)
  • Tetradecanoylphorbol Acetate (analogs & derivatives, immunology, metabolism)
  • Up-Regulation
  • Ziziphus (immunology)

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