Ethanol-induced oxidative stress: the role of binaphthyl diselenide as a potent antioxidant.

Abstract

It is widely accepted that oxidative stress plays a central role in alcohol-induced pathogenesis. The protective effect of binaphthyl diselenide (NapSe)2 was investigated in ethanol (Etoh)-induced brain injury. Thirty male adult Wistar rats were divided randomly into five groups of six animals each and treated as follows: (1) The control group received the vehicle (soy bean oil, 1 mL/kg, p.o.). (2) Ethanol group of animals was administered with ethanol (70% v/v, 2 mL/kg, p.o.). (3) (NapSe)2 1 mg/kg, 1 mL/kg plus ethanol 70% (v/v, 2 mL/kg, p.o. (5) (NapSe)2 10 mg/kg, 1 mL/kg) plus ethanol 70% (v/v, 2 mL/kg, p.o). After acute treatment, all rats were sacrificed by decapitation. Evidence for oxidative stress in rat brain was obtained from the observed levels of thiobarbituric acid reactive species, of non-protein thiol (NPSH) groups, and of ascorbic acid, as well as from the activities of catalase (CAT) and of superoxide dismutase (SOD). (NapSe)2 compensated the deficits in the antioxidant defense mechanisms (CAT, SOD, NPSH, and ascorbic acid), and suppressed lipid peroxidation in rat brain resulting from Etoh administration. It was concluded that ethanol exposure causes alterations in the antioxidant defense system and induces oxidative stress in rat brain. (NaPSe)2 at 5 mg/kg restored the antioxidant defenses in rat brain and mitigated the toxic effects of alcohol, suggesting that could be used as a potential therapeutic agent for alcohol-induced oxidative damage in rat brain.

• Authors: Mohammad Ibrahim, Waseem Hassan, Daiane Francine Meinerz, Gerlânia de Oliveira Leite, Cristina W Nogueira, Joao B T Rocha
• Journal: Biological trace element research (Biol Trace Elem Res) Vol. 147 Issue 1-3 Pg. 309-14 (Jun 2012) ISSN: 1559-0720 [Electronic] United States
• PMID: 22278096 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antioxidants
• Central Nervous System Depressants
• Organoselenium Compounds
• Sulfhydryl Compounds
• Thiobarbituric Acid Reactive Substances
• binaphthyl diselenide
• Ethanol
• Glutathione Peroxidase
• Superoxide Dismutase
• Ascorbic Acid

Topics

• Animals
• Antioxidants (pharmacology)
• Ascorbic Acid (metabolism)
• Brain (drug effects, metabolism)
• Central Nervous System Depressants (toxicity)
• Ethanol (toxicity)
• Glutathione Peroxidase (metabolism)
• Lipid Peroxidation (drug effects)
• Male
• Organoselenium Compounds (pharmacology)
• Oxidative Stress (drug effects)
• Random Allocation
• Rats
• Rats, Wistar
• Sulfhydryl Compounds (metabolism)
• Superoxide Dismutase (metabolism)
• Thiobarbituric Acid Reactive Substances (metabolism)