Immunohistochemical characterization of nonhuman primate ovarian sex cord-stromal tumors.

Abstract

This study evaluates the immunoreactivity of 12 sex cord-stromal tumors of nonhuman primates (11 granulosa cell tumors and 1 luteoma). The markers selected are used in the characterization of gonadal tumors in dogs and other species, including cytokeratins AE1/AE3, GATA-4, inhibin-α, neuron-specific enolase, protein gene product 9.5, and vimentin. A normal nonhuman primate ovary was used as a control and to optimize immunolabeling. Staining was graded as follows: 0 (nonstaining), 1+ (< 10% positive cells), 2+ (10%-50% positive cells), and 3+ (> 50% positive cells). Calretinin, GATA-4, neuron-specific enolase, and vimentin were the most consistently expressed markers (12 of 12). Cytokeratins AE1/AE3 were also consistently expressed (11 of 12). Inhibin-α and protein gene product 9.5 were expressed in 8 and 10 sex cord-stromal tumors, respectively. Results indicate that immunoreactivity of nonhuman primate sex cord-stromal tumors is similar to that observed in other species and that calretinin, GATA-4, and neuron-specific enolase are the most consistently expressed markers in nonhuman primate sex cord-stromal tumors.

• Authors: A Durkes, M Garner, C Juan-Sallés, J Ramos-Vara
• Journal: Veterinary pathology (Vet Pathol) Vol. 49 Issue 5 Pg. 834-8 (Sep 2012) ISSN: 1544-2217 [Electronic] United States
• PMID: 22273575 (Publication Type: Journal Article)

Chemical References

• Biomarkers, Tumor
• Calbindin 2
• Phosphopyruvate Hydratase

Topics

• Animals
• Biomarkers, Tumor (analysis, metabolism)
• Calbindin 2 (analysis, metabolism)
• Dogs
• Female
• Granulosa Cell Tumor (metabolism, pathology, veterinary)
• Humans
• Immunohistochemistry (veterinary)
• Luteoma (metabolism, pathology, veterinary)
• Ovarian Neoplasms (metabolism, pathology, veterinary)
• Phosphopyruvate Hydratase (analysis, metabolism)
• Primate Diseases (metabolism, pathology)
• Primates
• Sex Cord-Gonadal Stromal Tumors (metabolism, pathology, veterinary)