Thymoquinone have been shown to decrease
ischemia-reperfusion injury (IRI) in some tissues such as gastric mucosa and brain. In this study, the effect of
thymoquinone was evaluated on an animal model of IRI in the rat hind limb. Hind limb
ischemia was induced by clamping the common femoral artery and vein. After 2 h
ischemia, the clamp on the femoral vessels was taken off and the animal underwent 1 h reperfusion. Muscle
injuries were evaluated by recording the electromyographic (EMG) potentials and performing some biochemical analysis including
thiobarbituric acid reactive substances (
TBARS), total sulfhydryl (SH) groups, and
antioxidant capacity of muscle using ferric reducing ability of plasma (FRAP) assay.
Ischemia was induced using
free-flap surgery in skeletal muscle.
Thymoquinone (20, 40 and 80 mg/kg) and
normal saline (10 ml/kg) were administered intraperitoneally 1 h prior to reperfusion. The average peak-to-peak amplitude during ischemic reperfusion was significantly increased in
thymoquinone groups in comparison with the control group. Following
thymoquinone administration, the total SH contents and
antioxidant capacity were elevated in muscle flap. The
malondialdehyde (MDA) level was declined significantly in test groups. It is concluded that
thymoquinone have some protective effects against the muscle tissue injury caused by lower limb
ischemia-reperfusion.