Abstract | PURPOSE: MATERIALS AND METHODS: Mouse subcutaneous and lung metastasis bladder cancer models were established. Mice were injected subcutaneously with 1 × 10(6) mouse GM-CSF surface modified MB49 bladder cancer cells and monitored for tumor growth and survival. Immunohistochemical and flow cytometric assay were done to assess the proportion of T lymphocytes. The T-lymphocyte cytotoxicity assay was performed to assess MB49 specific cytotoxicity. On day 60 after MB49 implantation the vaccine cured mice were injected subcutaneously with MB49 or RM-1 cells in the left or right hind leg, respectively. They were monitored for survival and T-lymphocyte cytotoxicity. RESULTS: Mouse GM-CSF surface modified vaccine significantly inhibited tumor growth in the subcutaneous model and extended survival in the lung model. More CD4 and CD8 T cells appeared at tumor sites and in peripheral blood in the vaccine treated group than in other control groups. Splenocytes from the vaccine treated group showed the most potent cytotoxicity on MB49 cells. Cured mice in the vaccine treated group resisted the second injection of MB49 bladder cancer cells but not the RM-1 prostate cancer cell challenge. CONCLUSIONS:
|
Authors | Xinji Zhang, Xiaojun Shi, Jinlong Li, Zhiming Hu, Dong Zhou, Jimin Gao, Wanlong Tan |
Journal | The Journal of urology
(J Urol)
Vol. 187
Issue 3
Pg. 1071-9
(Mar 2012)
ISSN: 1527-3792 [Electronic] United States |
PMID | 22266013
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Copyright | Copyright © 2012 American Urological Association Education and Research, Inc. Published by Elsevier Inc. All rights reserved. |
Chemical References |
- Cancer Vaccines
- Granulocyte-Macrophage Colony-Stimulating Factor
- Streptavidin
|
Topics |
- Adaptive Immunity
- Analysis of Variance
- Animals
- Cancer Vaccines
(immunology)
- Cell Line, Tumor
- Female
- Flow Cytometry
- Granulocyte-Macrophage Colony-Stimulating Factor
(immunology)
- Immunohistochemistry
- Kaplan-Meier Estimate
- Lung Neoplasms
(secondary)
- Mice
- Mice, Inbred C57BL
- Neoplasm Invasiveness
(immunology)
- Streptavidin
- T-Lymphocytes, Cytotoxic
(immunology)
- Urinary Bladder Neoplasms
(immunology, pathology, prevention & control)
|