Immune modulation by Lacto-N-fucopentaose III in experimental autoimmune encephalomyelitis.

Abstract

Parasitic infections frequently lead to immune deviation or suppression. However, the application of specific parasitic molecules in regulating autoimmune responses remains to be explored. Here we report on the immune modulatory function of Lacto-N-fucopentaose III (LNFPIII), a schistosome glycan, in an animal model for multiple sclerosis. We found that LNFPIII treatment significantly reduced the severity of experimental autoimmune encephalomyelitis (EAE) and CNS inflammation, and skewed peripheral immune response to a Th2 dominant profile. Inflammatory monocytes (IMCs) purified from LNFPIII-treated mice had increased expression of nitric oxide synthase 2, and mediated T cell suppression. LNFPIII treatment also significantly increased mRNA expression of arginase-1, aldehyde dehydrogenase 1 subfamily A2, indoleamine 2,3-dioxygenase and heme oxygenase 1 in splenic IMCs. Furthermore, LNFPIII treatment significantly reduced trafficking of dendritic cells across brain endothelium in vitro. In summary, our study demonstrates that LNFPIII glycan treatment suppresses EAE by modulating both innate and T cell immune response.

• Authors: Bing Zhu, Subbulaxmi Trikudanathan, Alla L Zozulya, Carolina Sandoval-Garcia, Jennifer K Kennedy, Olga Atochina, Thomas Norberg, Bastien Castagner, Peter Seeberger, Zsuzsa Fabry, Donald Harn, Samia J Khoury, Indira Guleria
• Journal: Clinical immunology (Orlando, Fla.) (Clin Immunol) Vol. 142 Issue 3 Pg. 351-61 (Mar 2012) ISSN: 1521-7035 [Electronic] United States
• PMID: 22264636 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
• Copyright: Published by Elsevier Inc.

Chemical References

• Amino Sugars
• Polysaccharides
• lacto-N-fucopentaose III
• Nitric Oxide

Topics

• Amino Sugars (therapeutic use)
• Animals
• Cell Movement
• Dendritic Cells (cytology, immunology)
• Encephalomyelitis, Autoimmune, Experimental (drug therapy, immunology, pathology)
• Female
• Immunity, Innate
• Mice
• Mice, Inbred C57BL
• Nitric Oxide (biosynthesis, immunology)
• Phenotype
• Polysaccharides (therapeutic use)
• T-Lymphocytes (immunology)