Loss of lysosomal ion channel transient receptor potential channel mucolipin-1 (TRPML1) leads to cathepsin B-dependent apoptosis.

Abstract

Mucolipidosis type IV (MLIV) is a lysosomal storage disease caused by mutations in the gene MCOLN1, which codes for the transient receptor potential family ion channel TRPML1. MLIV has an early onset and is characterized by developmental delays, motor and cognitive deficiencies, gastric abnormalities, retinal degeneration, and corneal cloudiness. The degenerative aspects of MLIV have been attributed to cell death, whose mechanisms remain to be delineated in MLIV and in most other storage diseases. Here we report that an acute siRNA-mediated loss of TRPML1 specifically causes a leak of lysosomal protease cathepsin B (CatB) into the cytoplasm. CatB leak is associated with apoptosis, which can be prevented by CatB inhibition. Inhibition of the proapoptotic protein Bax prevents TRPML1 KD-mediated apoptosis but does not prevent cytosolic release of CatB. This is the first evidence of a mechanistic link between acute TRPML1 loss and cell death.

• Authors: Grace A Colletti, Mark T Miedel, James Quinn, Neel Andharia, Ora A Weisz, Kirill Kiselyov
• Journal: The Journal of biological chemistry (J Biol Chem) Vol. 287 Issue 11 Pg. 8082-91 (Mar 09 2012) ISSN: 1083-351X [Electronic] United States
• PMID: 22262857 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)

Chemical References

• BAX protein, human
• MCOLN1 protein, human
• Transient Receptor Potential Channels
• bcl-2-Associated X Protein
• CTSB protein, human
• Cathepsin B

Topics

• Cathepsin B (genetics, metabolism)
• Cytoplasm (genetics, metabolism, pathology)
• HeLa Cells
• Humans
• Lysosomes (genetics, metabolism, pathology)
• Mucolipidoses (genetics, metabolism, pathology)
• Transient Receptor Potential Channels (antagonists & inhibitors, genetics, metabolism)
• bcl-2-Associated X Protein (genetics, metabolism)