[Effect of recombinant trichosanthin on proliferation of human cevical cancer Caski cells].

Abstract

OBJECTIVE:

To observe the effects of high expression of recombinant trichosanthin (rTCS) on the cell proliferation and cell cycle of human cervical cancer Caski cells.

METHOD:

Eukaryotic expression plasmid pcDNA3.1(-)/6His-TCS was constracted and stably transfected into Caski cells. RT-PCR,Western-blot were used to select the clones with rTCS high-expressing. Using pcDNA3.1(-)-transfected cells as the control, MTT assay and flowcytometry were used to elucidate the effects of rTCS high expression on cell growth and cycle regulation in Caski cells.

RESULT:

The Caski cells with stable high expression of rTCS was successfully established, which could inhibit the cell growth (P<0.01) and arrest Caski cells in G1 and G2 phases (P<0.05) obviously.

CONCLUSION:

High expression of rTCS can inhibit the growth of Caski cervical cancer cells, which might provide a new pathway for the therapy of cervical cancer.

• Authors: Pingping Peng, Liming Huang, Yanlin Wang, Chengcheng You, Weihong Cao, Huamei Song, Hanxing Tan, Yanzhen Wu
• Journal: Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica (Zhongguo Zhong Yao Za Zhi) Vol. 36 Issue 18 Pg. 2539-42 (Sep 2011) ISSN: 1001-5302 [Print] China
• PMID: 22256762 (Publication Type: English Abstract, Journal Article)

Chemical References

• Recombinant Proteins
• Trichosanthin

Topics

• Cell Cycle (drug effects)
• Cell Line, Tumor
• Cell Proliferation (drug effects)
• Female
• Humans
• Recombinant Proteins (pharmacology)
• Transfection (methods)
• Trichosanthin (pharmacology)
• Uterine Cervical Neoplasms (pathology)