Abstract | OBJECTIVE: METHODS: We divided four groups of 249 male Wistar rats into nonimmersed controls, TP, and one (1-PTP) and three consecutive cycles (3-PTP) of PTP in a 42°C water bath. We evaluated the stress responses, including hemodynamics, total energy transfer, endoplasmic reticulum (ER) stress marker glucose-regulated protein ( GRP78), and blood reactive oxygen species level during TP or PTP treatment. We compared 1-PTP, 3-PTP, or TP effects on oxidative stress, intercellular adhesion molecule 1 (ICAM-1), Hsp70, tissue plasminogen activator (t-PA) and plasminogen activator inhibitor type 1 (PAI-1) activity, and vascular phosphorylated Akt (p-Akt) and eNOS (p-eNOS) expressions in a model of topical ferric chloride (FeCl(3))-induced carotid artery thrombosis. RESULTS: PTP significantly (P < .05) induced less hemodynamic fluctuations, total energy transfer, ER, and oxidative stress than TP did. After 24 or 72 hours of treatment, 1-PTP, 3-PTP, and TP significantly (P < .05) elevated carotid arterial Hsp70, p-Akt, and p-eNOS expression, significantly (P < .05) depressed FeCl(3)-enhanced vascular 2',7'-dichlorodihydrofluorescein diacetate, chemokine (C-X3-C motif) ligand 1 (CX3CL1), 3-nitrotyrosine, 4-hydroxynonenal, and ICAM-1 stain, PAI-1, and t-PA activity, leukocyte infiltration and thrombus size, and significantly (P < .05) delayed thrombus formation compared with controls. 3-PTP and TP had a higher (P < .05) protection than 1-PTP. PI3K/Akt, Hsp70, or N(G)-nitro-l-arginine methyl ester hydrochloride ( L-NAME) inhibitors significantly (P < .05) depressed 3-PTP and TP-induced vascular protection. CONCLUSIONS: Repetitive PTP is better than single PTP to hinder thrombosis formation via reinforcing PI3K/Akt-dependent Hsp70/eNOS signaling.
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Authors | Ping-Chia Li, Chih-Ching Yang, Shih-Ping Hsu, Chiang-Ting Chien |
Journal | Journal of vascular surgery
(J Vasc Surg)
Vol. 56
Issue 1
Pg. 159-70
(Jul 2012)
ISSN: 1097-6809 [Electronic] United States |
PMID | 22244861
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2012 Society for Vascular Surgery. Published by Mosby, Inc. All rights reserved. |
Chemical References |
- GRP78 protein, rat
- HSP70 Heat-Shock Proteins
- Heat-Shock Proteins
- Reactive Oxygen Species
- Nitric Oxide Synthase Type III
- Phosphatidylinositol 3-Kinase
- Proto-Oncogene Proteins c-akt
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Topics |
- Analysis of Variance
- Animals
- Carotid Stenosis
(metabolism, prevention & control)
- Disease Models, Animal
- Enzyme-Linked Immunosorbent Assay
- HSP70 Heat-Shock Proteins
(metabolism)
- Heat-Shock Proteins
(metabolism)
- Hemodynamics
- Hot Temperature
- Immunoblotting
- Male
- Nitric Oxide Synthase Type III
(metabolism)
- Oxidative Stress
- Phosphatidylinositol 3-Kinase
(metabolism)
- Proto-Oncogene Proteins c-akt
(metabolism)
- Rats
- Rats, Wistar
- Reactive Oxygen Species
(metabolism)
- Signal Transduction
- Thrombosis
(metabolism, prevention & control)
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