Biphasic influence of hypoxia on tuftelin expression in mouse mesenchymal C3H10T1/2 stem cells.

Abstract

Tuftelin, an acidic protein, thought to play a role in the initial stages of ectodermal enamel mineralization, has since been detected in mesenchymal-derived tissues. During bone/cartilage development and regeneration, mesenchymal stem cells (MSCs) undergo an avascular period in a hypoxic environment, involving induction of hypoxia-inducible factor 1-alpha (HIF-1-alpha), a key component in this process. In the present study we investigated, in a mouse mesenchymal C3H10T1/2 stem cell model, the hypothesis that oxygen stress modulates tuftelin 1 expression in relation to HIF-1-alpha (Hif1a), in a mouse mesenchymal C3H10T1/2 stem cell model. The results of the present study showed a biphasic induction of tuftelin, similar to the pattern of HIF-1-alpha expression, in MSCs subjected to a hypoxic insult of 1% O(2) through a period of 2-24 h. Immunocytochemistry analysis of the cells exposed to hypoxic insult for 2-24 h revealed the same biphasic pattern of tuftelin protein expression. Tuftelin localization appears to be mainly in the cytoplasm, and concentrated at the perinuclear region of the cells by 24 h of hypoxic insult. Based on our previous studies using the neuronal PC12 cell model, in which tuftelin induction was mediated by Hif1a, we propose that tuftelin is a member of oxygen-sensitive genes and implicated in the adaptive mechanisms regulating MSC function.

• Authors: Dan Deutsch, Nechama Silverstein, Dekel Shilo, Shimon Lecht, Philip Lazarovici, Anat Blumenfeld
• Journal: European journal of oral sciences (Eur J Oral Sci) Vol. 119 Suppl 1 Pg. 55-61 (Dec 2011) ISSN: 1600-0722 [Electronic] England
• PMID: 22243227 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2011 Eur J Oral Sci.

Chemical References

• Dental Enamel Proteins
• Hif1a protein, mouse
• Hypoxia-Inducible Factor 1, alpha Subunit
• tuftelin
• L-Lactate Dehydrogenase

Topics

• Adaptation, Physiological
• Animals
• Cell Death
• Cell Hypoxia (physiology)
• Cells, Cultured
• Dental Enamel Proteins (biosynthesis, genetics)
• Gene Expression Regulation, Developmental
• Hypoxia-Inducible Factor 1, alpha Subunit (biosynthesis, genetics)
• L-Lactate Dehydrogenase (metabolism)
• Mesenchymal Stem Cells (metabolism)
• Mice
• Mice, Inbred C3H