Stevioside ameliorates high-fat diet-induced insulin resistance and adipose tissue inflammation by downregulating the NF-κB pathway.

Abstract

Accumulating evidence suggests that adipose tissue is the main source of pro-inflammatory molecules that predispose individuals to insulin resistance. Stevioside (SVS) is a widely used sweetener with multiple beneficial effects for diabetic patients. In this study, we investigated the effect of SVS on insulin resistance and the pro-inflammatory state of adipose tissue in mice fed with a high-fat diet (HFD). Oral administration of SVS for 1month had no effect on body weight, but it significantly improved fasting glucose, basal insulin levels, glucose tolerance and whole body insulin sensitivity. Interestingly, these changes were accompanied with decreased expression levels of several inflammatory cytokines in adipose tissue, including TNF-α, IL6, IL10, IL1β, KC, MIP-1α, CD11b and CD14. Moreover, macrophage infiltration in adipose tissue was remarkably reduced by SVS. Finally, SVS significantly suppressed the nuclear factor-kappa b (NF-κB) signaling pathway in adipose tissue. Collectively, these results suggested that SVS may ameliorate insulin resistance in HFD-fed mice by attenuating adipose tissue inflammation and inhibiting the NF-κB pathway.

• Authors: Zhiquan Wang, Liqiong Xue, Cuicui Guo, Bing Han, Chunming Pan, Shuangxia Zhao, Huaidong Song, Qinyun Ma
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 417 Issue 4 Pg. 1280-5 (Jan 27 2012) ISSN: 1090-2104 [Electronic] United States
• PMID: 22240021 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2012 Elsevier Inc. All rights reserved.

Chemical References

• Cytokines
• Diterpenes, Kaurane
• Glucosides
• Insulin
• NF-kappa B
• RNA, Messenger
• Sweetening Agents
• stevioside

Topics

• Adipose Tissue (drug effects, pathology)
• Animals
• Cytokines (antagonists & inhibitors, biosynthesis)
• Diet, High-Fat (adverse effects)
• Diterpenes, Kaurane (administration & dosage)
• Down-Regulation
• Glucose Tolerance Test
• Glucosides (administration & dosage)
• Inflammation (drug therapy, metabolism, pathology)
• Insulin (pharmacology)
• Insulin Resistance
• Macrophages (drug effects)
• Male
• Mice
• Mice, Inbred C57BL
• NF-kappa B (antagonists & inhibitors, metabolism)
• RNA, Messenger (antagonists & inhibitors, biosynthesis)
• Sweetening Agents (administration & dosage)