Transient receptor potential
ankyrin 1 (TRPA1) is a
calcium-permeable non-selective
cation channel that is mainly expressed in primary nociceptive neurons. TRPA1 is activated by a variety of noxious stimuli, including cold temperatures, pungent compounds such as mustard oil and
cinnamaldehyde, and intracellular alkalization. Here, we show that primary
alcohols, which have been reported to cause skin, eye or nasal irritation, activate human TRPA1 (hTRPA1). We measured intracellular Ca(2+) changes in HEK293 cells expressing hTRPA1 induced by 1 mM primary
alcohols. Higher
alcohols (1-butanol to 1-octanol) showed Ca(2+) increases proportional to the
carbon chain length. In whole-cell patch-clamp recordings, higher
alcohols (1-hexanol to 1-octanol) activated hTRPA1 and the potency increased with the
carbon chain length. Higher
alcohols evoked single-channel opening of hTRPA1 in an inside-out configuration. In addition,
cysteine at 665 in the N terminus and
histidine at 983 in the C terminus were important for hTRPA1 activation by primary
alcohols. Furthermore, straight-chain secondary
alcohols increased intracellular Ca(2+) concentrations in HEK293 cells expressing hTRPA1, and both primary and secondary
alcohols showed hTRPA1 activation activities that correlated highly with their octanol/water partition coefficients. On the other hand, mouse TRPA1 did not show a strong response to
1-hexanol or
1-octanol, nor did these
alcohols evoke significant
pain in mice. We conclude that primary and secondary
alcohols activate hTRPA1 in a
carbon chain length-dependent manner. TRPA1 could be a sensor of
alcohols inducing skin, eye and nasal irritation in human.