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Human breast carcinoma cells are induced to apoptosis by samsum ant venom through an IGF-1-dependant pathway, PI3K/AKT and ERK signaling.

Abstract
In the present study we evaluated the anti-tumor potential of samsum ant venom (SAV) from Pachycondyla sennaarensis on the human breast carcinoma cell line MCF-7. We found that SAV induced growth arrest of MCF-7 cells without affecting the viability of MCF-10 (non-tumorigenic normal breast epithelial cells) and normal PBMCs. We then analyzed its impact on IGF-1-mediated MCF-7 cell proliferation and its effect on the underlying IGF-1 signaling pathways. Using flow cytometry analysis, we showed that the percentage of apoptotic cells was fourfold higher in SAV-treated cells as compared to untreated cells. More importantly, treatment with SAV induced a marked reduction in actin polymerization and a subsequent marked reduction in IGF-1-mediated cell proliferation. In addition to growth-inhibitory and pro-apoptotic effects, significant reductions were also observed in the phosphorylation of AKT and ERK, but not p38MAPK, in SAV-treated cells as compared to untreated cells. Our data reveal unique anti-tumor effects of samsum ant venom.
AuthorsGamal Badr, Olivier Garraud, Maha Daghestani, Mohammed Saleh Al-Khalifa, Yolande Richard
JournalCellular immunology (Cell Immunol) Vol. 273 Issue 1 Pg. 10-6 ( 2012) ISSN: 1090-2163 [Electronic] Netherlands
PMID22218396 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright © 2011 Elsevier Inc. All rights reserved.
Chemical References
  • Ant Venoms
  • Insulin-Like Growth Factor I
  • Phosphatidylinositol 3-Kinase
  • Proto-Oncogene Proteins c-akt
  • Extracellular Signal-Regulated MAP Kinases
Topics
  • Ant Venoms (pharmacology)
  • Apoptosis (drug effects)
  • Breast Neoplasms (drug therapy, enzymology, pathology)
  • Cell Growth Processes (drug effects)
  • Cell Line, Tumor
  • Cell Survival (drug effects)
  • Extracellular Signal-Regulated MAP Kinases (metabolism)
  • Female
  • Flow Cytometry
  • Humans
  • Immunoblotting
  • Insulin-Like Growth Factor I (metabolism)
  • MAP Kinase Signaling System
  • Phosphatidylinositol 3-Kinase (metabolism)
  • Phosphorylation
  • Proto-Oncogene Proteins c-akt (metabolism)

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