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Dextran sodium sulfate-induced inflammation alters the expression of proteins by intestinal Escherichia coli strains in a gnotobiotic mouse model.

Abstract
To identify Escherichia coli proteins involved in adaptation to intestinal inflammation, mice were monoassociated with the colitogenic E. coli strain UNC or with the probiotic E. coli strain Nissle. Intestinal inflammation was induced by treating the mice with 3.5% dextran sodium sulfate (DSS). Differentially expressed proteins in E. coli strains collected from cecal contents were identified by 2-dimensional difference gel electrophoresis. In both strains, acute inflammation led to the downregulation of pathways involved in carbohydrate breakdown and energy generation. Accordingly, DSS-treated mice had lower concentrations of bacterial fermentation products in their cecal contents than control mice. Differentially expressed proteins also included the Fe-S cluster repair protein NfuA, the tryptophanase TnaA, and the uncharacterized protein YggE. NfuA expression was 3-fold higher in E. coli strains from DSS-treated than from control mice. Reporter experiments confirmed the induction of nfuA in response to iron deprivation, mimicking Fe-S cluster destruction by inflammation. YggE expression, which has been reported to reduce the intracellular level of reactive oxygen species, was 4- to 8-fold higher in E. coli Nissle than in E. coli UNC. This was confirmed by in vitro reporter gene assays indicating that Nissle is better equipped to cope with oxidative stress than UNC. Nissle isolated from DSS-treated and control mice had TnaA levels 4- to 7-fold-higher than those of UNC. Levels of indole resulting from the TnaA reaction were higher in control animals associated with E. coli Nissle. Because of its anti-inflammatory effect, indole is hypothesized to be involved in the extension of the remission phase in ulcerative colitis described for E. coli Nissle.
AuthorsSara Schumann, Carl Alpert, Wolfram Engst, Gunnar Loh, Michael Blaut
JournalApplied and environmental microbiology (Appl Environ Microbiol) Vol. 78 Issue 5 Pg. 1513-22 (Mar 2012) ISSN: 1098-5336 [Electronic] United States
PMID22210207 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Escherichia coli Proteins
  • Dextran Sulfate
Topics
  • Animals
  • Cecum (chemistry, microbiology, pathology)
  • Dextran Sulfate (toxicity)
  • Electrophoresis, Gel, Two-Dimensional
  • Enteritis (chemically induced)
  • Escherichia coli (genetics, isolation & purification, metabolism)
  • Escherichia coli Proteins (biosynthesis, genetics)
  • Gene Expression Profiling
  • Germ-Free Life
  • Mice

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